Conformational cycle of the vitamin B12 ABC importer in liposomes detected by double electron-electron resonance (DEER).

The Journal of Biological Chemistry
Benesh JosephEnrica Bordignon

Abstract

Double electron-electron resonance is used here to investigate intermediates of the transport cycle of the Escherichia coli vitamin B12 ATP-binding cassette importer BtuCD-F. Previously, we showed the ATP-induced opening of the cytoplasmic gate I in TM5 helices, later confirmed by the AMP-PNP-bound BtuCD-F crystal structure. Here, other key residues are analyzed in TM10 helices (positions 307 and 322) and in the cytoplasmic gate II, i.e. the loop between TM2 and TM3 (positions 82 and 85). Without BtuF, binding of ATP induces detectable changes at positions 307 and 85 in BtuCD in liposomes. Together with BtuF, ATP triggers the closure of the cytoplasmic gate II in liposomes (reported by both positions 82 and 85). This forms a sealed cavity in the translocation channel in agreement with the AMP-PNP·BtuCD-F x-ray structure. When vitamin B12 and AMP-PNP are simultaneously present, the extent of complex formation is reduced, but the short 82-82 interspin distance detected indicates that the substrate does not affect the closed conformation of this gate. The existence of the BtuCD-F complex under these conditions is verified with spectroscopically orthogonal nitroxide and Gd(III)-based labels. The cytoplasmic gate II remains closed a...Continue Reading

References

Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Amy L DavidsonJue Chen
Oct 22, 2008·Journal of Bacteriology·Dmitry A RodionovThomas Eitinger
Oct 30, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Kaspar P Locher
Jul 8, 2009·Journal of Magnetic Resonance·René TschaggelarGunnar Jeschke
Sep 15, 2009·Trends in Biochemical Sciences·Peter M JonesAnthony M George
Feb 23, 2010·Nature Structural & Molecular Biology·Oded LewinsonDouglas C Rees
Jun 22, 2010·Nature Structural & Molecular Biology·Derek P ClaxtonHassane S McHaourab
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Cédric OrelleAmy L Davidson
Dec 1, 2010·Physical Chemistry Chemical Physics : PCCP·Yevhen PolyhachGunnar Jeschke
Sep 29, 2011·The Journal of Biological Chemistry·Benesh JosephEnrica Bordignon
Mar 1, 2012·Physical Chemistry Chemical Physics : PCCP·Ilia KaminkerDaniella Goldfarb
Mar 9, 2012·Journal of the American Chemical Society·Ute A HellmichClemens Glaubitz
Mar 13, 2012·Annual Review of Physical Chemistry·Gunnar Jeschke
Apr 24, 2012·The Journal of Biological Chemistry·Anshumali MittalMarkus A Seeger
Jun 30, 2012·Physical Chemistry Chemical Physics : PCCP·Maxim YulikovGunnar Jeschke
Jul 4, 2012·Physical Chemistry Chemical Physics : PCCP·Yevhen PolyhachGunnar Jeschke
Sep 25, 2012·Nature·Vladimir M KorkhovKaspar P Locher
Nov 13, 2012·Nature Structural & Molecular Biology·Jae-Sung WooKaspar P Locher
Jan 22, 2013·Nature Structural & Molecular Biology·Elka R GeorgievaOlga Boudker
Jan 22, 2013·Nature Structural & Molecular Biology·Inga HäneltDirk Jan Slotboom
Feb 5, 2013·The Journal of Physical Chemistry. B·Petra LuedersMaxim Yulikov
Feb 28, 2013·The Journal of Physical Chemistry. B·Luca GarbuioMaxim Yulikov
Mar 12, 2013·Chemistry and Physics of Lipids·Elizabeth J Denning, Oliver Beckstein
Mar 21, 2013·Proceedings of the National Academy of Sciences of the United States of America·Elena VigonskyOded Lewinson
May 28, 2013·The Journal of Biological Chemistry·Austin J RiceHeather W Pinkett
Jun 5, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·Gunnar Jeschke

❮ Previous
Next ❯

Citations

Nov 18, 2014·Nature Structural & Molecular Biology·Vladimir M KorkhovKaspar P Locher
Oct 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kelli KazmierHassane S Mchaourab
Oct 25, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alistair J FieldingMichael A Hollas
Jan 23, 2016·Journal of the American Chemical Society·Benesh JosephDavid S Cafiso
Nov 5, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ortal MarcianoSharon Ruthstein
Aug 27, 2014·Critical Reviews in Biochemistry and Molecular Biology·Austin J RiceHeather W Pinkett
Mar 31, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Yulia ShenbergerSharon Ruthstein
Jan 21, 2017·Journal of Molecular Biology·Oded Lewinson, Nurit Livnat-Levanon
May 26, 2017·Protein Science : a Publication of the Protein Society·Ariel R LevySharon Ruthstein
Jun 25, 2016·Physical Chemistry Chemical Physics : PCCP·Claudia E Tait, Stefan Stoll
Oct 7, 2016·Physical Chemistry Chemical Physics : PCCP·Katharina KellerMaxim Yulikov
Nov 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·Irene VercellinoVolodymyr M Korkhov
Dec 1, 2017·Physical Chemistry Chemical Physics : PCCP·Anna G MatveevaAlexander G Maryasov
Jun 20, 2018·Nature Chemical Biology·Min YangOded Lewinson
May 24, 2014·Channels·Austin J RiceHeather W Pinkett
Oct 21, 2016·Journal of Magnetic Resonance·Katharina KellerMaxim Yulikov
Aug 21, 2020·The Journal of Physical Chemistry Letters·Katja BarthBenesh Joseph

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.