PMID: 3763402Sep 25, 1986Paper

Conformational DNA transition in the in vitro torsionally strained chicken beta-globin 5' region

Nucleic Acids Research
L Runkel, A Nordheim

Abstract

A sequence of 86 bp within the 5' region of the adult chicken beta-globin gene was found to undergo a DNA conformational transition at elevated levels of negative superhelical stress (- sigma = 0.068). In vitro chemical DNA modification studies which detect purine hyperreactivity (HR) to the alkylating agent diethyl pyrocarbonate (DEP) have identified this 86 bp long DEP-HR element. The DEP-HR element is composed of small, tandem segments with imperfect purine-pyrimidine alternations. Methylation of cytosines within GCGC sequences of the DEP-HR element facilitates this structural change. The binding of a monoclonal anti-Z-DNA antibody to the element has been revealed by chemical footprinting with DEP. These data suggest that the DEP-HR sequence can undergo a conformational transition to Z-DNA. It is unknown whether the conformational flexibility observed here occurs in vivo.

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Citations

Aug 14, 1987·Biochemical and Biophysical Research Communications·Y Wang, W Sauerbier
Jan 31, 1989·Biochemical and Biophysical Research Communications·Y Wang, W Sauerbier
Sep 10, 2003·Journal of Molecular Biology·Mandy TamChristopher E Pearson
Feb 1, 1990·Journal of Molecular Recognition : JMR·P E Nielsen
Mar 28, 1997·Journal of Molecular Biology·C DaveyJ Allan
Feb 15, 1989·Biochemical and Biophysical Research Communications·J VlachJ Sponar

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