Conformational Epitope-Specific Broadly Neutralizing Plasma Antibodies Obtained from an HIV-1 Clade C-Infected Elite Neutralizer Mediate Autologous Virus Escape through Mutations in the V1 Loop

Journal of Virology
Shilpa PatilJayanta Bhattacharya

Abstract

Broadly neutralizing antibodies isolated from infected patients who are elite neutralizers have identified targets on HIV-1 envelope (Env) glycoprotein that are vulnerable to antibody neutralization; however, it is not known whether infection established by the majority of the circulating clade C strains in Indian patients elicit neutralizing antibody responses against any of the known targets. In the present study, we examined the specificity of a broad and potent cross-neutralizing plasma obtained from an Indian elite neutralizer infected with HIV-1 clade C. This plasma neutralized 53/57 (93%) HIV pseudoviruses prepared with Env from distinct HIV clades of different geographical origins. Mapping studies using gp120 core protein, single-residue knockout mutants, and chimeric viruses revealed that G37080 broadly cross-neutralizing (BCN) plasma lacks specificities to the CD4 binding site, gp41 membrane-proximal external region, N160 and N332 glycans, and R166 and K169 in the V1-V3 region and are known predominant targets for BCN antibodies. Depletion of G37080 plasma with soluble trimeric BG505-SOSIP.664 Env (but with neither monomeric gp120 nor clade C membrane-proximal external region peptides) resulted in significant reductio...Continue Reading

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Citations

Oct 25, 2017·Viral Immunology·Mahmoud Mohammad YaseenMohammad Mahmoud Yaseen
Mar 17, 2019·The Journal of Biological Chemistry·Rajaiah PerguSivaram V S Mylavarapu
Feb 23, 2020·Applied Microbiology and Biotechnology·Rajesh KumarHilal Ahmad Parray
May 18, 2019·BMC Veterinary Research·Juan Pablo JaworskiKarina Gabriela Trono
Feb 13, 2018·Vaccine·Tripti ShrivastavaBimal K Chakrabarti

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