PMID: 7543549Aug 1, 1995Paper

Conformational epitopes of pemphigus antigens (Dsg1 and Dsg3) are calcium dependent and glycosylation independent

The Journal of Investigative Dermatology
M AmagaiT Nishikawa

Abstract

The target molecule of pemphigus autoantibodies is a transmembrane desmosomal component, desmoglein 3 (Dsg3) in pemphigus vulgaris (PV) and Dsg1 in pemphigus foliaceus (PF). In this study, we examined the effects of calcium and glycosylation on the anti-genicity of the pemphigus antigens and on the generation of conformational epitopes. We used recombinant baculovirus proteins, PVIg and PFIg, which are considered to reflect accurately the native conformation of the extracellular domain of their respective proteins Dsg3 and Dsg1. These baculoproteins could immunoadsorb heterogeneous autoantibodies from the corresponding sera of PV and PF patients, completely blocking indirect immunofluorescence staining of normal human skin. Chelating calcium from the solution containing the baculoproteins using ethylenediaminetetraacetic acid (EDTA) or ethyleneglycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) abolished immunoadsorption by both PVIg and PFIg; however, immunoadsorption by the baculoproteins was restored after dialysis against 1 mM calcium. Nonglycosylated forms of both baculoproteins produced in the presence of tunicamycin retained their immunoadsorptive ability. Furthermore, immunoadsorption by the baculo-prote...Continue Reading

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Citations

Apr 16, 2002·Clinical and Experimental Dermatology·K E Harman
Apr 9, 2004·Nature Reviews. Molecular Cell Biology·Spiro GetsiosKathleen J Green
Dec 1, 2006·Autoimmunity·Sergei A Grando
Sep 26, 2019·Frontiers in Immunology·Sandra SaschenbreckerWolfgang Schlumberger
Mar 27, 2002·The Journal of Veterinary Medical Science·Rika MochizukiKohkichi Uehara
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Oct 13, 2011·The Journal of Pathology·Matthew A BrookeDavid P Kelsell
Nov 26, 2015·Seminars in Immunopathology·Giovanni Di ZenzoLuca Borradori
Nov 25, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Susana Ortiz-UrdaKlemens Rappersberger
Mar 20, 2004·Veterinary Dermatology·Wayne S Rosenkrantz
Jul 17, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ye QianLuis A Diaz
Mar 19, 2013·PloS One·Mina Kalantari-DehaghiSergei A Grando
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Dec 17, 2003·The Journal of Investigative Dermatology·Iakov ShimanovichDetlef Zillikens

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