Conformational features of a natural break in the type IV collagen Gly-X-Y repeat.

The Journal of Biological Chemistry
Angela MohsB Brodsky

Abstract

Fibrillar collagens have an absolute requirement for Gly as every 3rd residue, whereas breaks in the Gly-X-Y repeating pattern are found normally in the triple helix domains of non-fibrillar collagens, such as type IV collagen in basement membranes. In this study, a model 30-mer peptide is designed to include the interruption GPOGAAVMGPOGPO found in the alpha5 chain of type IV collagen. The GAAVM peptide forms a stable triple helix, with Tm= 29 degrees C. When compared with a control peptide with Gly as every 3rd residue, the GAAVM peptide has a marked decrease in the 225 nm maximum of its CD spectrum and a 10 degrees C drop in stability. A 50% decrease in calorimetric enthalpy is observed, which may result from disruption of ordered water structure anchored by regularly placed backbone carbonyls. NMR studies on specific 15N-labeled residues within the GAAVM peptide indicate a normal triple helical structure for Gly-Pro-Hyp residues flanking the break. The sequence within the break is not disordered but shows altered hydrogen exchange rates and an abnormal Val chemical shift. It was previously reported that a peptide designed to model a similar kind of interruption in the peptide (Pro-Hyp-Gly)10, (GPOGPOPOGPO), is unable to for...Continue Reading

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Citations

Aug 4, 2007·Journal of the American Chemical Society·Janelle Lauer-FieldsGregg B Fields
Jul 25, 2009·The Journal of Biological Chemistry·Jorge A FallasJeffrey D Hartgerink
Apr 30, 2008·Journal of Biochemistry·Hyeong Ju LeeHee Cheon Lee
Mar 6, 2010·Organic & Biomolecular Chemistry·Gregg B Fields
Mar 27, 2010·Protein Science : a Publication of the Protein Society·Eileen S HwangBarbara Brodsky
Apr 9, 2014·Journal of Structural Biology·Jordi Bella
Aug 3, 2010·Chemical Society Reviews·Jorge A FallasJeffrey D Hartgerink
Jun 25, 2014·The Journal of Biological Chemistry·Maciej J StawikowskiGregg B Fields
Apr 10, 2016·The Biochemical Journal·Jordi Bella
May 1, 2021·Bioengineering·Mahta MirzaeiAmin Shavandi
Jan 13, 2015·Biomacromolecules·Amanda M Acevedo-JakeJeffrey D Hartgerink

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