Conformational fluctuations in deoxy hemoglobin revealed as a major contributor to anionic modulation of function through studies of the oxygenation and oxidation of hemoglobins A0 and Deer Lodge beta2(NA2)His --> Arg

Biochemistry
Celia BonaventuraAlvin L Crumbliss

Abstract

Organisms rely on regulation at the molecular level, such as the allosteric regulation of hemoglobin (Hb) function by anions, to meet challenges presented by changing environmental and physiological conditions. A comparison of the effects of anions on oxygenation, oxidation, and sulfhydryl reactivity of Hb leads us to suggest that a large and significant part of the shift in oxygen affinity brought about by anion binding occurs as a result of increased conformational rigidity of the T state of deoxy Hb. As conformational rigidity increases, it becomes increasingly difficult for subunits in the deoxygenated T-state tetramer to assume higher oxygen affinity forms (T', T", T"'...) with less steric hindrance. The oxygen affinity reflects the average of the rapidly equilibrating conformations within the T state and is correspondingly decreased when anion levels are increased. The initial stage of the oxidation of Hb is relatively insensitive to steric alterations and thus reflects, primarily, the electronic aspects of the quaternary (T, T', T", T"'...) <--> equilibrium. We show that the reactivity of the sterically obscured sulfhydryl of beta93 Cys in deoxy Hb is much greater in chloride-free buffers than in buffers with added chlor...Continue Reading

References

Jan 3, 1992·Science·G K AckersM A Daugherty
Mar 1, 1990·Biophysical Journal·M C MardenC Poyart
May 1, 1989·Quarterly Reviews of Biophysics·M F Perutz
Oct 1, 1987·Biophysical Journal·J KisterS J Edelstein
Dec 14, 1972·Journal of Molecular Biology·A Szabo, M Karplus
Aug 1, 1973·The Biochemical Journal·J V Kilmartin
Mar 1, 1972·Clinical Biochemistry·A LabossiereP Galbraith
Mar 1, 1967·Archives of Biochemistry and Biophysics·D R Grassetti, J F Murray
Jan 15, 1980·Journal of Molecular Biology·M F Perutz, K Imai
Jun 17, 1994·Journal of Molecular Biology·M F PerutzD Williamson
Aug 15, 1997·Journal of Molecular Biology·M PaoliA J Wilkinson
Mar 20, 1956·The Journal of General Physiology·A F RIGGS, R A WOLBACH
May 1, 1965·Journal of Molecular Biology·J MONODJ P CHANGEUX

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Citations

Oct 15, 2003·Biophysical Chemistry·Weilin PengGenxi Li
May 6, 1999·Bioelectrochemistry and Bioenergetics·C H TaboyA L Crumbliss
Nov 19, 2004·Antioxidants & Redox Signaling·Celia BonaventuraAlvin L Crumbliss
Dec 17, 2009·Analytical Biochemistry·Zhibin MaiXiaoyong Zou
Oct 20, 2004·Acta Physiologica Scandinavica·C BonaventuraR E Weber
Aug 24, 1999·The Journal of Biological Chemistry·C BonaventuraR D Stevens
Sep 14, 2000·The Journal of Biological Chemistry·C H TaboyA L Crumbliss
Mar 20, 1999·The Journal of Biological Chemistry·C BonaventuraJ Friedman
Sep 21, 2006·The Journal of Biological Chemistry·Camille J RocheJoel M Friedman
Mar 29, 2001·Physiological Reviews·R E Weber, S N Vinogradov
Dec 17, 2002·Blood Cells, Molecules & Diseases·Qiuying ChenRhoda Elison Hirsch

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