Conformational memory in the association of the transmembrane protein phospholamban with the sarcoplasmic reticulum calcium pump SERCA

The Journal of Biological Chemistry
Serena SmeazzettoFrancesco Tadini-Buoninsegni

Abstract

The sarcoplasmic reticulum Ca2+-ATPase SERCA promotes muscle relaxation by pumping calcium ions from the cytoplasm into the sarcoplasmic reticulum. SERCA activity is regulated by a variety of small transmembrane peptides, most notably by phospholamban in cardiac muscle and sarcolipin in skeletal muscle. However, how phospholamban and sarcolipin regulate SERCA is not fully understood. In the present study, we evaluated the effects of phospholamban and sarcolipin on calcium translocation and ATP hydrolysis by SERCA under conditions that mimic environments in sarcoplasmic reticulum membranes. For pre-steady-state current measurements, proteoliposomes containing SERCA and phospholamban or sarcolipin were adsorbed to a solid-supported membrane and activated by substrate concentration jumps. We observed that phospholamban altered ATP-dependent calcium translocation by SERCA within the first transport cycle, whereas sarcolipin did not. Using pre-steady-state charge (calcium) translocation and steady-state ATPase activity under substrate conditions (various calcium and/or ATP concentrations) promoting particular conformational states of SERCA, we found that the effect of phospholamban on SERCA depends on substrate preincubation conditi...Continue Reading

References

Jan 1, 1979·Annual Review of Biochemistry·L de Meis, A L Vianna
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G B WarrenJ C Metcalfe
Sep 1, 1972·Biochimica Et Biophysica Acta·S Eletr, G Inesi
Dec 5, 1998·The Journal of Biological Chemistry·G ChuE G Kranias
Jan 14, 1999·The American Journal of Physiology·Y JiM Periasamy
Aug 9, 2002·Nature·Chikashi Toyoshima, Hiromi Nomura
Jan 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Chikashi ToyoshimaDavid H MacLennan
Jun 11, 2004·Biophysical Journal·Francesco Tadini BuoninsegniRolando Guidelli
Feb 22, 2005·Protein Expression and Purification·Jennifer L DouglasHoward S Young
Sep 15, 2005·Journal of the American Chemical Society·Ovidiu C AndronesiMarc Baldus
Oct 13, 2006·The Journal of Biological Chemistry·Francesco Tadini-BuoninsegniGiuseppe Inesi
Nov 18, 2008·Biochimica Et Biophysica Acta·Chikashi Toyoshima
Aug 22, 2009·The Journal of Biological Chemistry·Daniele CatalucciGianluigi Condorelli
Sep 3, 2010·Quarterly Reviews of Biophysics·Jesper V MøllerPoul Nissen
Jan 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Chikashi ToyoshimaShiho Iwasawa
Jun 21, 2011·Physical Chemistry Chemical Physics : PCCP·Serena SmeazzettoMaria Rosa Moncelli
Aug 13, 2011·The Journal of Biological Chemistry·Philip BidwellSeth L Robia
Mar 20, 2012·The Journal of Biological Chemistry·Delaine K CeholskiHoward S Young
Sep 11, 2012·Nature Medicine·Naresh C BalMuthu Periasamy
Jan 31, 2013·The Journal of Biological Chemistry·Przemek A GorskiHoward S Young
Mar 5, 2013·Nature·Anne-Marie L WintherMorten J Buch-Pedersen
Jul 23, 2013·Current Opinion in Structural Biology·Chikashi Toyoshima, Flemming Cornelius
Sep 3, 2013·The Journal of Biological Chemistry·Brandy L AkinLarry R Jones
Oct 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Martin GustavssonGianluigi Veglia
Jan 17, 2015·The Journal of Biological Chemistry·Przemek A GorskiHoward S Young
Oct 1, 2015·The Journal of Physical Chemistry. B·Mario SchörnerJürgen Köhler
Oct 6, 2015·Scientific Reports·Francesco Tadini-BuoninsegniPierandrea Lo Nostro
Sep 23, 2016·Biophysical Journal·Daniel J BlackwellSeth L Robia

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Citations

Sep 13, 2019·Chemical Biology & Drug Design·Kiran RahateKedar S Prabhavalkar
Jun 14, 2020·International Journal of Molecular Sciences·Rodrigo Aguayo-Ortiz, L Michel Espinoza-Fonseca
Sep 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Francesco Tadini-Buoninsegni
Oct 19, 2020·Biophysical Journal·Olga N RaguimovaL Michel Espinoza-Fonseca
Aug 28, 2021·International Journal of Molecular Sciences·Nishadh RathodHoward S Young
Jul 24, 2021·ChemMedChem·Giacomo SordiFrancesco Tadini-Buoninsegni

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