Conformational ordering of biomolecules in the gas phase: nitrogen collision cross sections measured on a prototype high resolution drift tube ion mobility-mass spectrometer

Analytical Chemistry
Jody C MayJohn A McLean

Abstract

Ion mobility-mass spectrometry measurements which describe the gas-phase scaling of molecular size and mass are of both fundamental and pragmatic utility. Fundamentally, such measurements expand our understanding of intrinsic intramolecular folding forces in the absence of solvent. Practically, reproducible transport properties, such as gas-phase collision cross-section (CCS), are analytically useful metrics for identification and characterization purposes. Here, we report 594 CCS values obtained in nitrogen drift gas on an electrostatic drift tube ion mobility-mass spectrometry (IM-MS) instrument. The instrument platform is a newly developed prototype incorporating a uniform-field drift tube bracketed by electrodynamic ion funnels and coupled to a high resolution quadrupole time-of-flight mass spectrometer. The CCS values reported here are of high experimental precision (±0.5% or better) and represent four chemically distinct classes of molecules (quaternary ammonium salts, lipids, peptides, and carbohydrates), which enables structural comparisons to be made between molecules of different chemical compositions for the rapid "omni-omic" characterization of complex biological samples. Comparisons made between helium and nitrogen...Continue Reading

References

Oct 28, 1999·Journal of the American Society for Mass Spectrometry·S J ValentineD E Clemmer
Aug 17, 2001·Journal of the American Society for Mass Spectrometry·A A ShvartsburgD E Clemmer
Apr 16, 2002·Journal of the American Society for Mass Spectrometry·Laura M MatzIsik Kanik
Sep 24, 2004·Rapid Communications in Mass Spectrometry : RCM·Kevin GilesRobert H Bateman
Oct 4, 2005·Rapid Communications in Mass Spectrometry : RCM·Jyrki ViidanojaTapio Kotiaho
Nov 19, 2005·Science·Brandon T RuotoloCarol V Robinson
Jul 15, 2006·Journal of the American Society for Mass Spectrometry·Yehia IbrahimRichard D Smith
Nov 14, 2006·Nucleic Acids Research·Manish SudShankar Subramaniam
May 22, 2007·Journal of the American Society for Mass Spectrometry·Lei TaoDavid H Russell
May 22, 2007·Journal of the American Society for Mass Spectrometry·Erin Shammel BakerRichard D Smith
Sep 14, 2007·Analytical Chemistry·Yehia IbrahimRichard D Smith
Mar 6, 2008·Analytical and Bioanalytical Chemistry·Larissa S Fenn, John A McLean
Jul 5, 2008·Nature Protocols·Brandon T RuotoloCarol V Robinson
Feb 28, 2009·Analytical and Bioanalytical Chemistry·Larissa S FennJohn A McLean
Aug 4, 2009·Journal of the American Society for Mass Spectrometry·John A McLean
Nov 30, 2010·Physical Chemistry Chemical Physics : PCCP·Larissa S Fenn, John A McLean
Aug 1, 2012·Analytical Chemistry·Matthew F BushCarol V Robinson
Mar 16, 2013·The Journal of Physical Chemistry. a·Carlos Larriba, Christopher J Hogan

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Citations

Feb 13, 2016·Journal of the American Society for Mass Spectrometry·Joshua A SilveiraMelvin A Park
Nov 26, 2015·Journal of Chromatography. a·Michael A EwingDavid E Clemmer
Dec 3, 2014·Current Opinion in Biotechnology·Jody C MayJohn A McLean
Nov 4, 2015·International Journal for Ion Mobility Spectrometry : Official Publication of the International Society for Ion Mobility Spectrometry·Paolo BenigniFrancisco Fernandez-Lima
Apr 3, 2016·Analytical and Bioanalytical Chemistry·Susanne StephanOliver J Schmitz
Jul 25, 2015·Journal of the American Society for Mass Spectrometry·David J HarveyJim H Scrivens
Aug 12, 2015·Journal of Mass Spectrometry : JMS·Valentina D'AtriValérie Gabelica
Apr 18, 2015·International Journal of Mass Spectrometry·Joseph A HankinMiguel A Gijón
Jul 18, 2015·International Journal of Mass Spectrometry·Yehia M IbrahimRichard D Smith
Apr 22, 2015·Analytical and Bioanalytical Chemistry·Giuseppe PagliaGiuseppe Astarita
Jun 23, 2016·International Journal for Ion Mobility Spectrometry : Official Publication of the International Society for Ion Mobility Spectrometry·Emily R SchenkFrancisco Fernandez-Lima
Sep 20, 2016·International Journal for Ion Mobility Spectrometry : Official Publication of the International Society for Ion Mobility Spectrometry·Kendra J AdamsFrancisco Fernandez-Lima
Mar 6, 2015·Frontiers in Microbiology·Kristian F Nielsen, Thomas O Larsen
Oct 22, 2014·Journal of the American Society for Mass Spectrometry·Karsten MichelmannMelvin A Park
May 2, 2016·Current Opinion in Biotechnology·Jody Christopher MayJohn Allen McLean
Jun 17, 2016·Annual Review of Analytical Chemistry·Jody C May, John A McLean
Aug 9, 2016·Analytical and Bioanalytical Chemistry·Susanne StephanOliver J Schmitz
Nov 1, 2016·Journal of the American Society for Mass Spectrometry·Samuel J Allen, Matthew F Bush
Nov 4, 2016·Journal of Mass Spectrometry : JMS·David J HarveyJames H Scrivens
Dec 16, 2016·Journal of the American Society for Mass Spectrometry·Julia L Kaszycki, Alexandre A Shvartsburg
Mar 17, 2017·Nature Protocols·Giuseppe Paglia, Giuseppe Astarita
Apr 25, 2017·Angewandte Chemie·Johanna Hofmann, Kevin Pagel
Jan 21, 2017·International Journal of Molecular Sciences·Roza WojcikRichard D Smith

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Software Mentioned

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