Conformational preferences of a chimeric peptide HIV-1 immunogen from the C4-V3 domains of gp120 envelope protein of HIV-1 CAN0A based on solution NMR: comparison to a related immunogenic peptide from HIV-1 RF

Biochemistry
H M VuL D Spicer

Abstract

A critical problem to overcome on HIV vaccine design is the variability among HIV strains. One strategy to solve this problem is the construction of multicomponent immunogens reflective of common HIV motifs. Currently, it is not known if these motifs should be based primarily on amino acid sequence or higher-order structure of the viral proteins of a combination of the two. In this paper, we report NMR-derived solution conformations for a sympathetic peptide taken from the C4 and V3 domains of HIV-1 CAN0A gp120 envelope protein. This peptide, designated T1-SP10CAN0(A), is compared to a recently reported C4-V3 peptide. T1-SP10RF(A) from the HIV-1 RF strain [de Lorimier et al. (1994) Biochemistry 33, 2055-2062], in terms of conformational features and immune responses in mice [Haynes et al. (1995) AIDS Res. Hum. Retroviruses 11, 211-221]. The T1 segment of 16 amino acids from the gp120 C4 domain is identical in both peptides and exhibits nascent helical character. The SP10 region, taken from the gp120 V3 loop, differs from that of T1-SP10RF(A) in both sequence and conformations. A reverse turn is observed at the conserved GPGX sequence. The rest of the Sp10 domain is extended with the exception of the last three residues which sh...Continue Reading

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Citations

Mar 27, 1997·Journal of Biochemical and Biophysical Methods·A V SarmaA C Kunwar
Sep 6, 2011·Journal of Chemical Information and Modeling·Alexander M AndrianovAlexander V Tuzikov
Dec 25, 2003·Journal of Biomolecular Structure & Dynamics·Alexander M Andrianov, Yuri A Sokolov
Aug 20, 2004·Journal of Biomolecular Structure & Dynamics·Alexander M Andrianov
Oct 13, 2005·Journal of Biomolecular Structure & Dynamics·A M Andrianov
Aug 15, 2012·Journal of Biomolecular Structure & Dynamics·Alexander M AndrianovAlexander V Tuzikov
Nov 30, 2005·Journal of Molecular Modeling·Joel K WeltmanGeorge B Loriot
Dec 11, 2002·The Journal of Peptide Research : Official Journal of the American Peptide Society·M JasejaJ Starkey

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