Conformational preferences of linear beta-defensins are revealed by ion mobility-mass spectrometry.

The Journal of Physical Chemistry. B
Martin De CeccoPerdita Barran

Abstract

In recent times there has been an enormous rise in resistance to synthetic antibiotics as well as an increase in the virulence of bacteria, the so-called "superbugs". This problem has catalyzed a search for novel molecules to fight bacteria, which in turn relies on a better understanding of the molecular basis of the immune response. Beta-defensins are a class of small, cationic, cysteine-rich antimicrobial peptides expressed by humans and other animals to act against incoming pathogens. As well as their antimicrobial properties, beta-defensins also act as chemokines, recruiting cells to the sites of infection. Here the relationship between the tertiary structures of beta-defensin analogs and their chemotactic activities has been investigated using ion mobility-mass spectrometry (IM-MS) and biochemical assays. A panel of derivatives of the murine beta-defensin Defb14 has been formed and the ability of these peptides to chemoattract the receptor CCR6 has been assessed in vitro. The derivatives can be divided into two groups, those with chemotactic activity equal to that of the unmodified parent peptide, and those whose chemotactic activity has been lost upon modification. Analysis by ion mobility-mass spectrometry reveals the co...Continue Reading

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Citations

Sep 19, 2012·Journal of Proteome Research·Ross ChawnerClaire E Eyers
Jul 8, 2011·International Journal of Antimicrobial Agents·Nicole K Brogden, Kim A Brogden
Jul 7, 2015·Rapid Communications in Mass Spectrometry : RCM·Kevin Jeanne Dit FouqueFabien Chirot
Jun 24, 2015·Journal of Peptide Science : an Official Publication of the European Peptide Society·Xibo TianLong Chen
Jun 15, 2011·Methods : a Companion to Methods in Enzymology·Sophie R HarveyPerdita E Barran
Sep 21, 2010·Chemical Communications : Chem Comm·Yana BerezovskayaPerdita E Barran
Jun 30, 2018·Organic & Biomolecular Chemistry·Helena Martin-Gómez, Judit Tulla-Puche

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