Conformational reorientation of immunoglobulin G during nonspecific interaction with surfaces

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
Michael RanklStefan Seeger

Abstract

To achieve a better understanding of the nonspecific adsorption process of proteins on solid surfaces, the mechanism of this interaction was investigated by a model system comprising the structurally flexible ("soft") protein goat anti-rabbit immunoglobulin G and a set of chemically defined surfaces. The thermodynamic properties of both protein and surfaces were derived from contact angle measurements by applying the Lifshitz-van der Waals acid-base approach, and the Gibbs free enthalpy of interaction was calculated. The protein shows two conformational states, one hydrophobic and the other hydrophilic. The interaction energy indicates that the hydrophobic conformation favorably adsorbs onto the surfaces. With real-time binding kinetics, measured by a supercritical angle fluorescence biosensor, we show that during the nonspecific adsorption the protein performs a reorientation in its three-dimensional amino acid structure from a hydrophilic to a hydrophobic molecular structure. Unlike the rates of adsorption and desorption, the transition rate is independent of the type of surface and only influenced by the structural reorganization of the protein.

References

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Citations

Aug 5, 2010·Journal of the American Chemical Society·David J NiedzwieckiLiviu Movileanu
Oct 13, 2012·Journal of Chromatography. a·Samantha SchachermeyerWenwan Zhong
Mar 28, 2007·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Michael RabeStefan Seeger
Jan 31, 2008·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Marco Nonella, Stefan Seeger
May 4, 2006·Chembiochem : a European Journal of Chemical Biology·Stefan JungStefan Seeger
Oct 15, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·He HuangXiaohu Wu
Feb 8, 2011·Advances in Colloid and Interface Science·Michael RabeStefan Seeger
Jun 24, 2020·Journal of the Royal Society, Interface·Victor AkpeIan E Cock
Oct 16, 2018·Frontiers in Microbiology·Evelína KánováMangesh Bhide
Aug 5, 2017·Frontiers in Bioengineering and Biotechnology·Ziwei HuangGil Garnier
Mar 2, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Nazia MehrbanChristopher O'Callaghan
May 23, 2008·Analytical Chemistry·Mark LowryIsiah M Warner
Oct 10, 2008·The Journal of Physical Chemistry. B·Michael RabeStefan Seeger
Dec 25, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Jan ZimmermannStefan Seeger

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