PMID: 39067Jul 1, 1979

Conformational stability of ribonuclease T1. I. Thermal denaturation and effects of salts

Journal of Biochemistry
M OobatakeT Ooi

Abstract

The thermal transition of RNase T1 was studied by two different methods; tryptophan residue fluorescence and circular dichroism. The fluorescence measurements provide information about the environment of the indole group and CD measurements on the gross conformation of the polypeptide chain. Both measurements at pH 5 gave the same transition temperature of 56 degrees C and the same thermodynamic quantities, delta Htr (= 120 kcal/mol) and delta Str (= 360 eu/mol), for the transition from the native state to the thermally denatured state, indicating simultaneous melting of the whole molecule including the hydrophobic region where the tryptophan residue is buried. Stabilization by salts was observed in the pH range from 2 to 10, since the presence of 0.5 m NaCL caused an increase of about 5 degrees C to 10 degrees C in the transition temperature, depending on the pH. The fluorescence measurements on the RNase T1 complexed with 2'-GMP showed a transition with delta Htr =167 kcal/mol and delta Str =497 eu/mol at a transition temperature about 6 degrees C higher than that for the free enzyme. The large value of delta Htr for RNase T1 indicates the highly cooperative nature of the thermal transition; this value is much higher than tho...Continue Reading

Related Concepts

Circular Dichroism, Vibrational
Drug Stability
RNase Pch 1
Hydrogen-Ion Concentration
Osmolality
Protein Conformation
Protein Denaturation
Alkaline Ribonuclease
Sodium Chloride, (24)NaCl
Fluorescence Spectroscopy

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