PMID: 7543023Jul 28, 1995Paper

Conformational states of CFTR associated with channel gating: the role ATP binding and hydrolysis

Cell
K L Gunderson, R R Kopito

Abstract

CFTR is a member of the traffic ATPase superfamily and a Cl- ion channel that appears to require ATP hydrolysis for gating. Analysis of single CFTR Cl- channels reconstituted into planar lipid bilayers revealed the presence of two open conductance states that are connected to each other and to the closed state by an asymmetric cycle of gating events. We show here that the transition between the two open conductance states is directly coupled to ATP hydrolysis by one of the consensus nucleotide-binding folds, designated NBF2. Moreover, the transition between the closed state and one of the open states is linked to the binding of ATP. This analysis permits real-time visualization of conformational changes associated with a single cycle of ATP hydrolysis by a single protein molecule and suggests a model describing a role for ATP in CFTR gating.

References

Jun 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G F Ames, H Lecar
Feb 5, 1992·Journal of Molecular Biology·M Kjeldgaard, J Nyborg
Nov 1, 1990·Trends in Biochemical Sciences·M SarasteA Wittinghofer
Mar 1, 1990·Research in Microbiology·C F Higgins
Jan 1, 1980·Annual Review of Biochemistry·J R Knowles
Mar 1, 1994·Neuron·T BaukrowitzD C Gadsby
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·T C HwangD C Gadsby
Nov 1, 1994·Trends in Biochemical Sciences·D C Gadsby, A C Nairn
Apr 1, 1993·The American Journal of Physiology·C L Bell, P M Quinton

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Citations

Nov 2, 2001·Biochemical and Biophysical Research Communications·F Kembi, M A Harrington
Aug 4, 2004·Pflügers Archiv : European journal of physiology·Tomohiko AiTzyh-Chang Hwang
Oct 6, 2006·Pflügers Archiv : European journal of physiology·Andrei A AleksandrovJohn R Riordan
Jul 16, 2010·The Journal of Physiological Sciences : JPS·Hiroyasu ShimizuYoshiro Sohma
Apr 5, 2011·The Journal of Physiological Sciences : JPS·Oleg V KrasilnikovYasunobu Okada
Jun 1, 1996·Current Opinion in Genetics & Development·M T Keating, M C Sanguinetti
Jun 1, 1996·Current Opinion in Neurobiology·M S Sonders, S G Amara
Jun 1, 1996·Current Opinion in Neurobiology·T J Jentsch
Mar 24, 2006·Nature·David C GadsbyLászló Csanády
Sep 29, 2004·Nature Structural & Molecular Biology·Christopher F Higgins, Kenneth J Linton
Oct 26, 2005·Biochemical Society Transactions·P VerganiD C Gadsby
Sep 21, 2000·European Journal of Biochemistry·T Baukrowitz, B Fakler
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·D J HennagerM J Welsh
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·László CsanádyDavid C Gadsby
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Christopher Miller
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·J T KarttunenP Cresswell
Jul 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·M Ikuma, M J Welsh
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·P DrainJ Wang
Aug 31, 2006·International Journal of Toxicology·Akira TakahashiYutaka Nakaya
Jan 1, 1996·The Journal of General Physiology·D J WilkinsonD C Dawson
Jul 10, 1999·The Journal of General Physiology·L Csanády, D C Gadsby
May 30, 2002·The Journal of General Physiology·Athanasios G DousmanisDavid C Gadsby
Jan 1, 2003·The Journal of General Physiology·Paola VerganiDavid C Gadsby
Nov 12, 2003·The Journal of General Physiology·Xiandi Gong, Paul Linsdell
Jan 20, 2005·The Journal of General Physiology·Wei WangKevin L Kirk
Mar 16, 2005·The Journal of General Physiology·Silvia G BompadreTzyh-Chang Hwang
Oct 18, 2006·The Journal of General Physiology·László CsanádyDavid C Gadsby
Apr 1, 2009·The Journal of General Physiology·Ming-Feng TsaiTzyh-Chang Hwang
May 18, 2011·The Journal of General Physiology·Andras SzollosiPaola Vergani
Apr 18, 2012·The Journal of General Physiology·Kang-Yang JihTzyh-Chang Hwang
Sep 12, 2012·The Journal of General Physiology·Kang-Yang JihTzyh-Chang Hwang
Sep 12, 2012·The Journal of General Physiology·Ming-Feng Tsai
Oct 30, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Daniella Muallem, Paola Vergani

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