Conformational states of the severe acute respiratory syndrome coronavirus spike protein ectodomain

Journal of Virology
Fang LiStephen C Harrison

Abstract

The severe acute respiratory syndrome coronavirus enters cells through the activities of a spike protein (S) which has receptor-binding (S1) and membrane fusion (S2) regions. We have characterized four sequential states of a purified recombinant S ectodomain (S-e) comprising S1 and the ectodomain of S2. They are S-e monomers, uncleaved S-e trimers, cleaved S-e trimers, and dissociated S1 monomers and S2 trimer rosettes. Lowered pH induces an irreversible transition from flexible, L-shaped S-e monomers to clove-shaped trimers. Protease cleavage of the trimer occurs at the S1-S2 boundary; an ensuing S1 dissociation leads to a major rearrangement of the trimeric S2 and to formation of rosettes likely to represent clusters of elongated, postfusion trimers of S2 associated through their fusion peptides. The states and transitions of S suggest conformational changes that mediate viral entry into cells.

References

Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R K WilliamsK V Holmes
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P L EarlB Moss
Dec 1, 1988·The Journal of General Virology·W SpaanM C Horzinek
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J J SkehelD C Wiley
Feb 1, 1994·Trends in Microbiology·H D Klenk, W Garten
Aug 31, 2000·Annual Review of Biochemistry·J J Skehel, D C Wiley
Jun 8, 2001·Annual Review of Biochemistry·D M Eckert, P S Kim
Apr 12, 2003·The New England Journal of Medicine·Thomas G KsiazekUNKNOWN SARS Working Group
Apr 25, 2003·Lancet·J S M PeirisUNKNOWN SARS study group
May 6, 2003·Science·Marco A MarraRachel L Roper
Dec 4, 2003·Biochemical and Biophysical Research Communications·Xiaodong XiaoDimiter S Dimitrov
Dec 13, 2003·The Journal of Biological Chemistry·Swee Kee WongMichael Farzan
Mar 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Graham SimmonsPaul Bates
Sep 4, 2004·The Journal of Biological Chemistry·Yanhui XuZihe Rao
Apr 16, 2005·Science·Kartik ChandranJames M Cunningham
Aug 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Graham SimmonsPaul Bates
Aug 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Shutoku MatsuyamaFumihiro Taguchi
Dec 13, 2005·The Journal of Biological Chemistry·I-Chueh HuangHyeryun Choe

❮ Previous
Next ❯

Citations

Feb 10, 2009·Nature Reviews. Microbiology·Lanying DuShibo Jiang
Mar 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sandrine BelouzardGary R Whittaker
Feb 1, 2012·The Journal of Biological Chemistry·Kailang WuFang Li
Oct 24, 2012·The Journal of Biological Chemistry·Guiqing PengFang Li
Jul 13, 2007·Journal of Virology·Patricia EifartAndreas Herrmann
Oct 31, 2008·Journal of Virology·Miyuki KawaseFumihiro Taguchi
Nov 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sarah A ConnollyRobert A Lamb
Mar 5, 2016·Traffic·Judith M White, Gary R Whittaker
Dec 3, 2014·Virus Research·Jean Kaoru Millet, Gary R Whittaker
Sep 1, 2016·Annual Review of Virology·Fang Li
Oct 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Alexandra C WallsDavid Veesler
Jun 24, 2008·Critical Reviews in Biochemistry and Molecular Biology·Judith M WhiteKathryn Schornberg
Mar 5, 2016·Nature·Robert N KirchdoerferAndrew B Ward
Dec 13, 2019·Journal of Virology·Yushun WanFang Li
Jul 24, 2020·Journal of Biomolecular Structure & Dynamics·Preeti PandeyShashikant Ray
Jul 31, 2020·Archives of Physiology and Biochemistry·Heena Rehman, Md Iftekhar Ahmad
Oct 13, 2016·The Journal of Biological Chemistry·Chang LiuFang Li
Dec 12, 2018·Nature Reviews. Microbiology·Jie CuiZheng-Li Shi
May 8, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jian ShangFang Li
Jul 28, 2020·DNA and Cell Biology·Arghavan AsghariNegin Parsamanesh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.