Conformations of gas-phase ions of ubiquitin, cytochrome c, apomyoglobin, and beta-lactoglobulin produced from two different solution conformations.

Journal of the American Society for Mass Spectrometry
P John WrightD J Douglas

Abstract

At low pH in solutions of 50% methanol, proteins form expanded denatured states (the "H" state). In 90% methanol, proteins form expanded helical denatured states with artificial alpha-helices (the "H(c)" state). Gas-phase ions of ubiquitin, cytochrome c, apomyoglobin, and native and disulfide-reduced beta-lactoglobulin were formed by electrospray ionization (ESI) of the proteins from the H and H(c) states in solution. Both states in solution produce the same charge states in ESI. The conformations of the ions were studied with cross section measurements and gas-phase H/D exchange experiments. The cross sections show that the ions retain considerable folded structure. For a given protein and given charge state, ions produced from the H and H(c) states showed the same cross sections (within approximately 1%). Ions of cytochrome c, apomyoglobin, and native and reduced beta-lactoglobulin of a given charge state showed no differences in H/D exchange level when produced from the H or H(c) state. However, ubiquitin ions produced from the H(c) state consistently exchange fewer ( approximately 13%) hydrogens than ions produced from the H state, suggesting that in this case the gas-phase protein ions retain some memory of their solution ...Continue Reading

References

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Citations

Oct 19, 2011·Journal of the American Society for Mass Spectrometry·Yang KangD J Douglas
Dec 23, 2008·Journal of the American Society for Mass Spectrometry·P John Wright, D J Douglas
Mar 17, 2010·Journal of the American Society for Mass Spectrometry·Jonathan P WilliamsPeter J Sadler
Apr 1, 2011·The Journal of Physical Chemistry. B·Brian C BohrerDavid E Clemmer
Mar 12, 2016·Journal of the American Society for Mass Spectrometry·Shaolong ZhuDerek J Wilson
Jul 1, 2015·Journal of the American Chemical Society·Michael B CammarataJennifer S Brodbelt
Oct 17, 2014·Journal of the American Society for Mass Spectrometry·Khadijeh Rajabi
May 6, 2015·Physical Chemistry Chemical Physics : PCCP·Liudmila Voronina, Thomas R Rizzo
May 20, 2017·Rapid Communications in Mass Spectrometry : RCM·Kenichiro FurukiHideto Yamaguchi
Nov 11, 2011·Physical Chemistry Chemical Physics : PCCP·Anne MaisserChristopher J Hogan
Jan 1, 2015·Journal of Mass Spectrometry : JMS·Yury KostyukevichEugene Nikolaev

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