PMID: 8611645Jan 17, 1996Paper

Conformations of synthetic beta peptides in solid state and in aqueous solution: relation to toxicity in PC12 cells

Biochimica Et Biophysica Acta
René BuchetD A Lowe

Abstract

The secondary structures of peptides beta 25-35 (the active toxic fragment) and beta 35-25 (reverse sequence and non-toxic fragment), as well as of the amidated beta (25-35)-NH2 peptide were investigated in aqueous solution and in the solid state by means of Fourier-transformed infrared spectroscopy and circular dichroism spectroscopy. The conformations of the beta 25-35 and beta 35-25 in solid state were identical and contained mostly beta-sheet structures. In solid state the amidated beta (25-35)-NH2 peptide also contained mostly beta-sheet structures. Freshly prepared aqueous solutions of the beta 25-32 (0.5 - 3.8 mM) contained a mixture of beta-sheet and random coil structures. Within 30-60 min incubation at 37 degrees C in water or in phosphate-buffered saline solution (PBS), beta 25-35 was almost fully converted to a beta-sheet structure. Decreasing the temperature from 37 degrees C to 20 degrees C decreased the rate of conversion from random coil to beta-sheet structures, 1-2 h being required for complete conversion. In contrast beta 35-25 in water or in PBS buffer had mostly a random coil structure and remained so for 6 days. The amidated beta(25-35)-NH2 peptide in water (2.7 mM) was also mostly random coil. However, wh...Continue Reading

References

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Citations

Oct 20, 1999·Journal of Molecular Neuroscience : MN·J B StrosznajderR P Strosznajder
May 3, 2002·European Journal of Biochemistry·Raghda LahdoLaurence de La Fournière-Bessueille
Feb 14, 1998·European Journal of Biochemistry·L de La Fournière-BessueilleR Buchet
Aug 11, 2005·Biochemical and Biophysical Research Communications·Ganesh ShanmugamZsuzsa Majer
Oct 7, 2004·Biopolymers·Ganesh Shanmugam, Rajadas Jayakumar
May 19, 2007·Journal of Neuroscience Research·Emanuela CazzanigaMassimo Masserini
Apr 29, 1998·The Journal of Biological Chemistry·M PappollaJ Ghiso

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