Conformations of variably linked chimeric proteins evaluated by synchrotron X-ray small-angle scattering

Proteins
Ryoichi AraiTetsuro Fujisawa

Abstract

We constructed chimeric proteins that consist of two green fluorescent protein variants, EBFP and EGFP, connected by flexible linkers, (GGGGS)n (n = 3 approximately 4), and helical linkers, (EAAAK)n (n = 2 approximately 5). The conformations of the chimeric proteins with the various linkers were evaluated using small-angle X-ray scattering (SAXS). The SAXS experiments showed that introducing the short helical linkers (n = 2 approximately 3) causes multimerization, while the longer linkers (n = 4 approximately 5) solvate monomeric chimeric proteins. With the moderate-length linkers (n = 4), the observed radius of gyration (R(g)) and maximum dimension (D(max)) were 38.8 A and 120 A with the flexible linker, and 40.2 A and 130 A with the helical linker, respectively. The chimeric protein with the helical linker assumed a more elongated conformation as compared to that with the flexible linker. When the length of the helical linker increased (n = 5), R(g) and D(max) increased to 43.2 A and 140 A, respectively. These results suggest that the longer helix effectively separates the two domains of the chimeric protein. Considering the connectivity of the backbone peptide of the protein, the helical linker seems to connect the two domai...Continue Reading

References

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Marqusee, R L Baldwin
Oct 21, 1988·Science·R E BirdM Whitlow
Apr 1, 1994·Analytical Biochemistry·P Wu, L Brand
Jul 1, 1995·Protein Engineering·K AlfthanT T Teeri
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Jul 1, 1997·Analytical Biochemistry·Y MaedaT Nagamune
Jan 22, 1998·Current Opinion in Structural Biology·G N Phillips
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·D I SvergunG Zaccai
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·C R Robinson, R T Sauer
Oct 1, 1996·Nature Biotechnology·F YangG N Phillips
Oct 6, 1998·Annual Review of Biochemistry·R Y Tsien
May 1, 1999·Journal of Structural Biology·W WriggersJ A McCammon
Jun 2, 2000·Protein Engineering·C J Crasto, J A Feng
Jul 15, 2000·The Journal of Biological Chemistry·S S FunariD I Svergun

❮ Previous
Next ❯

Citations

May 10, 2005·Biopolymers·Willy WriggersPatricia A Jennings
May 16, 2012·Analytical and Bioanalytical Chemistry·Ranieri BizzarriFabio Beltram
Apr 17, 2008·Applied Microbiology and Biotechnology·Ping Lu, Ming-Guang Feng
Nov 13, 2008·Pharmaceutical Research·Nurmamet AmetWei-Chiang Shen
May 6, 2011·The Journal of Biological Chemistry·Kenneth P HolbournK Ravi Acharya
Oct 10, 2008·The Journal of Biological Chemistry·Jermaine L JenkinsClara L Kielkopf
Nov 10, 2011·Journal of Biomolecular Structure & Dynamics·Alessio LombardiMaria Sperandei
Sep 3, 2011·Antioxidants & Redox Signaling·Masahide Oku, Yasuyoshi Sakai
Sep 29, 2011·International Journal of Molecular Sciences·Anton P Le BrunJeremy H Lakey
Jul 17, 2010·Bioinformatics and Biology Insights·Christian Gorba, Florence Tama
Oct 3, 2012·Advanced Drug Delivery Reviews·Xiaoying ChenWei-Chiang Shen
Oct 23, 2014·Organic & Biomolecular Chemistry·Dung T DangLuc Brunsveld
May 9, 2009·Journal of Cell Communication and Signaling·Kenneth P HolbournK Ravi Acharya
Feb 19, 2016·Chemical Reviews·Elena PapaleoRuth Nussinov
Sep 24, 2015·Applied Microbiology and Biotechnology·Gang LiXin-Hui Xing
Oct 28, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yue-Jin WuYaw-Kuen Li
Apr 13, 2007·Progress in Neurobiology·Matthias Gralle, Sérgio T Ferreira
May 24, 2011·Archives of Biochemistry and Biophysics·Tracy D TurbevilleGloria C Ferreira
Aug 20, 2015·BMC Biotechnology·Gro Elin Kjæreng Bjerga, Adele Kim Williamson
Sep 29, 2015·Iranian Journal of Cancer Prevention·Abbas Ali Imani FooladiJafar Amani
Sep 21, 2010·Journal of Structural Biology·Osamu MiyashitaFlorence Tama
Jun 9, 2016·The International Journal of Biochemistry & Cell Biology·Longguang JiangMingdong Huang
May 12, 2017·Nano Convergence·Teruyuki Nagamune
Jan 29, 2008·Experimental Biology and Medicine·Vladimir L KolossovH Rex Gaskins
May 2, 2018·Journal of Bacteriology·Catarina G FernandesAdriano O Henriques
Jul 26, 2014·The Journal of Biological Chemistry·Carter J Swanson, Sivaraj Sivaramakrishnan
Feb 26, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nobutaka MatsumuraHiroshi Yanagawa
Jul 24, 2012·Toxins·Frank SainsburyDominique Michaud
Nov 16, 2016·Frontiers in Cellular and Infection Microbiology·Víctor M Luna-PinedaJuan Xicohtencatl-Cortes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.