Confronting the constraints of morphological taxonomy in the Botryosphaeriales

Persoonia
Bernard SlippersG J Marais

Abstract

Identification of fungi and the International Code of Nomenclature underpinning this process, rests strongly on the characterisation of morphological structures. Yet, the value of these characters to define species in many groups has become questionable or even superfluous. This has emerged as DNA-based techniques have increasingly revealed cryptic species and species complexes. This problem is vividly illustrated in the present study where 105 isolates of the Botryosphaeriales were recovered from both healthy and diseased woody tissues of native Acacia spp. in Namibia and South Africa. Thirteen phylogenetically distinct groups were identified based on Internal Transcribed Spacer (ITS) rDNA PCR-RFLP and translation elongation factor 1-α (TEF1-α) sequence data, two loci that are known to be reliable markers to distinguish species in the Botryosphaeriales. Four of these groups could be linked reliably to sequence data for formerly described species, including Botryosphaeria dothidea, Dothiorella dulcispinae, Lasiodiplodia pseudotheobromae and Spencermartinsia viticola. Nine groups, however, could not be linked to any other species known from culture and for which sequence data are available. These groups are, therefore, described...Continue Reading

Citations

Feb 20, 2016·Antonie van Leeuwenhoek·Milica ZlatkovićBernard Slippers
Jan 18, 2014·International Journal of Systematic and Evolutionary Microbiology·Lopamudra RayVishakha Raina
Jan 31, 2020·Plant Disease·Xiang-Rong ZhengFeng-Mao Chen
Aug 19, 2020·Molecular Ecology Resources·Lua LopezJesse R Lasky
Nov 27, 2017·EFSA Journal·UNKNOWN EFSA Panel on Plant Health (PLH)Vittorio Rossi
Aug 24, 2021·Fungal Biology·María VivasBernard Slippers

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Datasets Mentioned

BETA
MB518717
MB518718
MB518721
MB518723
MB518719
MB518722
MB518720
MB518724

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BLAST
TreeBASE
MAFFT
PAUP
Modeltest
Sequence Navigator
Axiovision

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