Congressing kinetochores progressively load Ska complexes to prevent force-dependent detachment

The Journal of Cell Biology
Philip AucklandAndrew D McAinsh

Abstract

Kinetochores mediate chromosome congression by either sliding along the lattice of spindle microtubules or forming end-on attachments to their depolymerizing plus-ends. By following the fates of individual kinetochores as they congress in live cells, we reveal that the Ska complex is required for a distinct substep of the depolymerization-coupled pulling mechanism. Ska depletion increases the frequency of naturally occurring, force-dependent P kinetochore detachment events, while being dispensable for the initial biorientation and movement of chromosomes. In unperturbed cells, these release events are followed by reattachment and successful congression, whereas in Ska-depleted cells, detached kinetochores remain in a futile reattachment/detachment cycle that prevents congression. We further find that Ska is progressively loaded onto bioriented kinetochore pairs as they congress. We thus propose a model in which kinetochores mature through Ska complex recruitment and that this is required for improved load-bearing capacity and silencing of the spindle assembly checkpoint.

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Citations

Mar 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·Luke A HelgesonTrisha N Davis
Jan 24, 2018·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Qian ZhangHong Liu
Nov 27, 2018·ELife·Claudia PellacaniMaria Patrizia Somma
Feb 10, 2019·Biomolecules·Margarida Moura, Carlos Conde
May 26, 2018·Nature Communications·Damian DudkaPatrick Meraldi
May 30, 2019·Journal of Cell Science·Banafsheh EtemadGeert J P L Kops
May 24, 2019·The Journal of Cell Biology·Karthik DhatchinamoorthyJennifer L Gerton
Mar 25, 2020·The Journal of Cell Biology·Philip AucklandAndrew D McAinsh
Aug 28, 2020·Essays in Biochemistry·Amit RahiDileep Varma
May 22, 2020·The Journal of Cell Biology·Alexandra F LongSophie Dumont
Mar 13, 2020·Frontiers in Cell and Developmental Biology·Robert T Wimbish, Jennifer G DeLuca
Aug 18, 2018·Journal of Cell Science·Julie K Monda, Iain M Cheeseman
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Mar 24, 2021·Seminars in Cell & Developmental Biology·Alexandra P Navarro, Iain M Cheeseman
Apr 4, 2021·International Journal of Molecular Sciences·Ayumu Yamamoto

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transfection
PCR
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Software Mentioned

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Gatan
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