Conifer WOX-related homeodomain transcription factors, developmental consideration and expression dynamic of WOX2 during Picea abies somatic embryogenesis.

Plant Molecular Biology
Joakim Palovaara, Inger Hakman

Abstract

In angiosperms, the WOX family of transcription factors has important functions in meristem regulation and in control of the partitioning of developing embryos into functional domains. In this study, a putative WOX2 homologous gene was isolated from Picea abies, and its expression pattern during somatic embryo development was followed using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). We used strategies of both absolute and relative quantification of gene expression, and benefits and disadvantages of the two methods are presented and discussed. During embryogenesis, PaWOX2 expression was highest at the earliest stages of development, but low levels were also detected in seedling tissues. No PaWOX2 expression was detected in a non-embryogenic cell culture, indicating that PaWOX2 plays a fundamental role during early somatic embryo development, and can be used as a possible marker for embryogenic potential. Additional results show that conifers, like angiosperms, contain a large number of WOX-related genes, many of them expressed during embryo development. In phylogenetic analysis based on the deduced homeodomain of retrieved pine and spruce EST sequences, no conifer WUS homolog was found. Nei...Continue Reading

References

Jun 5, 1987·Science·W J Gehring
Dec 15, 1993·Gene·W J Gehring
Feb 1, 1996·Current Opinion in Structural Biology·C Wolberger
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jan 5, 2000·Journal of Biotechnology·O ThellinE Heinen
Jul 12, 2001·The EMBO Journal·G Jürgens
Jul 27, 2001·Nucleic Acids Research·S Banerjee-Basu, A D Baxevanis
Feb 19, 2002·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Katleen De PreterJo Vandesompele
Apr 20, 2002·Science·Nigel J Walker
Apr 1, 2003·Journal of Cell Science·Rita Gross-Hardt, Thomas Laux
Aug 9, 2003·Nucleic Acids Research·R G Rutledge, C Côté
Sep 6, 2003·Journal of Immunological Methods·Joseph A WhelanMichael A Whelan
Oct 8, 2003·Nucleic Acids Research·Ales TichopadMichael W Pfaffl
Jan 17, 2004·Clinical Chemistry·Anders StåhlbergMikael Kubista
Mar 24, 2004·Molecular and Cellular Probes·Ales TichopadMichael W Pfaffl
Apr 22, 2004·The Plant Cell·Thomas LauxHolger Breuninger
Dec 1, 2004·Annual Review of Genetics·Viola Willemsen, Ben Scheres
Jan 18, 2005·Current Opinion in Plant Biology·Dolf Weijers, Gerd Jürgens
Apr 5, 2005·Nature Genetics·Markus SchmidJan U Lohmann
Apr 9, 2005·Genes and Immunity·J HuggettA Zumla
Nov 3, 2005·The Plant Journal : for Cell and Molecular Biology·Silvia GonzaliPierdomenico Perata
Nov 30, 2005·Nucleic Acids Research·Oleg Suslov, Dennis A Steindler
Dec 6, 2005·BMC Developmental Biology·Karen GoossensLuc J Peelman
Dec 13, 2006·Journal of Experimental Botany·Nijat IminBarry G Rolfe
Mar 6, 2007·Plant Physiology·Sandra RichardtStefan A Rensing
Apr 5, 2007·Nature Protocols·Tania NolanStephen A Bustin
Sep 5, 2007·Molecular Biology and Evolution·Judith NardmannWolfgang Werr

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Citations

Jan 14, 2010·Genome Biology·Eric van der GraaffStefan A Rensing
Mar 14, 2014·Journal of Experimental Botany·Andrei Smertenko, Peter V Bozhkov
Feb 7, 2009·Plant Physiology and Biochemistry : PPB·Kensuke Kawade, Kiyoshi Masuda
Dec 4, 2012·Physiologia Plantarum·Gregory A GambettaMark A Matthews
Nov 22, 2011·Plant Physiology and Biochemistry : PPB·Anita WiśniewskaMarcin Filipecki
Nov 24, 2016·International Journal of Molecular Sciences·Yali LiuLiwang Qi
Apr 7, 2012·Genetics and Molecular Biology·Paulo Sérgio SchlöglMiguel Pedro Guerra
Mar 7, 2019·Frontiers in Plant Science·Isabel ArrillagaEster Sales
Nov 15, 2018·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Shatrujeet PandeyPratibha Misra
Jul 6, 2014·BMC Genomics·Qingfen LiJunhui Wang
Sep 5, 2021·Physiologia Plantarum·Liyang SuBingcong Xing

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