PMID: 8955281Dec 1, 1996Paper

Conjugal transfer of the 5-nitroimidazole resistance plasmid pIP417 from Bacteroides vulgatus BV-17: characterization and nucleotide sequence analysis of the mobilization region

Journal of Bacteriology
S TrinhG Reysset

Abstract

Three small 5-nitroimidazole (5-Ni) resistance plasmids (pIP417, pIP419, and pIP421) from Bacteroides clinical isolates are transferable by a conjugative process during homologous or heterologous matings. The mobilization properties of pIP417 originated from strain BV-17 of Bacteroides vulgatus were studied. The plasmid was successfully introduced by in vitro conjugation into different strains of Bacteroides and Prevotella species and could be transferred back from these various strains to a plasmid-free 5-Ni-sensitive Bacteroides fragilis strain, indicating that in vivo spread of the resistance gene may occur. The transfer of plasmid pIP417 harbored by the Tc(r) strain BF-2 of B. fragilis was stimulated by low concentrations of tetracycline or chlorotetracycline. This suggests a possible role for coresident conjugative transposons in the dissemination of 5-Ni resistance among gram-negative anaerobes. The nucleotide sequence of the 2.1-kb DNA mobilization region was determined. It contains a putative origin of transfer (oriT) in an A+T-rich-region, including three inverted repeats, and two integration host factor binding sites. The two identified mobilization genes (mobA and mobB) are organized in one operon and were both requi...Continue Reading

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Citations

Oct 28, 2017·The Journal of Antimicrobial Chemotherapy·Simon A Dingsdag, Neil Hunter
Dec 13, 2005·The Journal of Antimicrobial Chemotherapy·József SókiBrian I Duerden
Mar 18, 1999·FEMS Microbiology Letters·M M LubbeL J Chalkley
Jul 10, 2001·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·A HaggoudM Moumni
May 16, 2002·Periodontology 2000·Marilyn C Roberts
Feb 25, 2005·Antimicrobial Agents and Chemotherapy·Sonja LöfmarkCharlotta Edlund
Sep 12, 1998·Journal of Bacteriology·L A SitailoD W Hecht
Jul 11, 1998·Plasmid·C J SmithD P Bayley

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