Conjugated docosahexaenoic acid suppresses KPL-1 human breast cancer cell growth in vitro and in vivo: potential mechanisms of action

Breast Cancer Research : BCR
Miki Tsujita-KyutokuAiro Tsubura

Abstract

The present study was conducted to examine the effect of conjugated docosahexaenoic acid (CDHA) on cell growth, cell cycle progression, mode of cell death, and expression of cell cycle regulatory and/or apoptosis-related proteins in KPL-1 human breast cancer cell line. This effect of CDHA was compared with that of docosahexaenoic acid (DHA). KPL-1 cell growth was assessed by colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; cell cycle progression and mode of cell death were examined by flow cytometry; and levels of expression of p53, p21Cip1/Waf1, cyclin D1, Bax, and Bcl-2 proteins were examined by Western blotting analysis. In vivo tumor growth was examined by injecting KPL-1 cells subcutaneously into the area of the right thoracic mammary fat pad of female athymic mice fed a CDHA diet. CDHA inhibited KPL-1 cells more effectively than did DHA (50% inhibitory concentration for 72 hours: 97 micromol/l and 270 micromol/l, respectively). With both CDHA and DHA growth inhibition was due to apoptosis, as indicated by the appearance of a sub-G1 fraction. The apoptosis cascade involved downregulation of Bcl-2 protein; Bax expression was unchanged. Cell cycle progression was due to G0/G1 arrest, which inv...Continue Reading

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Citations

May 12, 2005·Journal of Mammary Gland Biology and Neoplasia·Airo TsuburaNobuaki Shikata
Dec 17, 2010·Lipids·Alan A HennessyCatherine Stanton
Aug 26, 2006·The International Journal of Biochemistry & Cell Biology·Banu IsbilenMustafa B A Djamgoz
Aug 18, 2017·International Journal of Molecular Sciences·Marnie NewellCatherine J Field
Aug 11, 2007·Bioscience, Biotechnology, and Biochemistry·Tsuyoshi TsuzukiTeruo Miyazawa
Mar 13, 2016·Lipids·Alan A HennessyCatherine Stanton
May 10, 2007·Molecular and Cellular Biochemistry·Hamid ZandSaideh Salimi
Dec 10, 2020·Biomarker Research·Zhaoqing LiJichun Zhou

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Methods Mentioned

BETA
chips
flow cytometry

Software Mentioned

Cell Quest
Modfit
NIH Image1

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