Conjugated linoleic acid isomers modulate protein expression profile in rat hepatocytes.

Genes & Nutrition
E RossiA Iannone

Abstract

Conjugated linoleic acid (CLA) is a polyunsaturated fatty acid, which has been recently proven to be effective in reducing body fat mass, but brings as a side effect, the liver enlargement due to an increased lipid content. The in vivo lipogenic activity has been suggested to be due to the reduction in fat mass and to the consequent metabolism of blood glucose to fatty acid in the liver rather than in the adipose tissue. We investigated the ability of CLA to directly induce steatosis by modulating the expression pattern of hepatic proteins involved in lipid metabolism. To avoid interferences derived from CLA metabolism by other tissues, we used the in vitro model of freshly isolated rat hepatocytes incubated in the presence of different CLA isomers. The direct effect of CLA on lipid accumulation in hepatocytes was demonstrated by the altered expression pattern of several proteins involved in lipid metabolism, as assessed by two-dimensional gel electrophoresis and confirmed by Western blotting analysis. The CLA isomer c9,t11 was most effective in modulating the protein expression profile.

References

Oct 15, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·S Landaas
Jun 1, 1978·Chemical & Pharmaceutical Bulletin·M Yamaguchi, T Yamamoto
Oct 1, 1990·Archives of Biochemistry and Biophysics·N IwataT Satoh
Feb 1, 1981·Chemical & Pharmaceutical Bulletin·M Yamaguchi, K Sugii
Jan 1, 1994·Advances in Enzyme Regulation·F M Huennekens
Jul 1, 1994·Atherosclerosis·K N LeeM W Pariza
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Aug 1, 1996·Virchows Archiv : an International Journal of Pathology·C W WuS R Wang
Aug 15, 1998·Biochemical and Biophysical Research Communications·K N LeeJ M Ntambi
Jan 16, 1999·The Journal of Biological Chemistry·M ArakiM Ikeda
May 16, 2000·Life Sciences·M Yamaguchi
Sep 28, 2000·Biochemical and Biophysical Research Communications·M Yamaguchi
May 1, 2001·The Journal of Laboratory and Clinical Medicine·S S GökmenS Gülen
May 29, 2001·Current Medicinal Chemistry·J G Liehr, J S Jones
Jul 19, 2001·The Journal of Biological Chemistry·J M Olefsky
Jul 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·L H WeiL J Ignarro
Aug 4, 2001·The American Journal of Pathology·F ChenX Shi
Apr 6, 2002·The Journal of Biological Chemistry·K Aftab RashidSteven L Chuck
May 3, 2002·Blood·Frank M Torti, Suzy V Torti
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Diane R WonseyChi V Dang
Oct 3, 2002·Nature Reviews. Immunology·Qiutang Li, Inder M Verma
Dec 20, 2002·Nature·Carl Nathan
Dec 21, 2002·European Journal of Biochemistry·Megumi MasakiToshisuke Kawasaki
Dec 25, 2002·Medicinal Research Reviews·Claudiu T SupuranAngela Casini
Dec 31, 2002·Biochemical and Biophysical Research Communications·Polkit SangvanichMichael Mackness
Jan 10, 2003·Journal of Gastroenterology and Hepatology·Takuya MiyagiNorio Hayashi
Jun 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Tao SuI Bernard Weinstein
Apr 3, 2004·Journal of Lipid Research·Marleen M J Van GreevenbroekTjerk W A De Bruin
May 18, 2004·Biochemical and Biophysical Research Communications·Bharti MacknessMike Mackness
May 26, 2004·The American Journal of Clinical Nutrition·Jean-Michel GaullierOla Gudmundsen

❮ Previous
Next ❯

Citations

Nov 17, 2016·Omics : a Journal of Integrative Biology·Camila Pereira BragaPedro de Magalhães Padilha
Jul 11, 2021·International Journal of Pharmaceutics·Lindokuhle M NgemaYahya E Choonara

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.