Conjugated Polymer-Based Hybrid Nanoparticles with Two-Photon Excitation and Near-Infrared Emission Features for Fluorescence Bioimaging within the Biological Window

ACS Applied Materials & Interfaces
Yanlin LvZhiyuan Tian

Abstract

Hybrid fluorescent nanoparticles (NPs) capable of fluorescing near-infrared (NIR) light (centered ∼730 nm) upon excitation of 800 nm laser light were constructed. A new type of conjugated polymer with two-photon excited fluorescence (TPEF) feature, P-F8-DPSB, was used as the NIR-light harvesting component and the energy donor while a NIR fluorescent dye, DPA-PR-PDI, was used as the energy acceptor and the NIR-light emitting component for the construction of the fluorescent NPs. The hybrid NPs possess δ value up to 2.3 × 10(6) GM per particle upon excitation of 800 nm pulse laser. The excellent two-photon absorption (TPA) property of the conjugated polymer component, together with its high fluorescence quantum yield (ϕ) up to 45% and the efficient energy transfer from the conjugated polymer to NIR-emitting fluorophore with efficiency up to 90%, imparted the hybrid NPs with TPEF-based NIR-input-NIR-output fluorescence imaging ability with penetration depth up to 1200 μm. The practicability of the hybrid NPs for fluorescence imaging in Hela cells was validated.

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Citations

Dec 13, 2017·Materials·Yasmine BraekenWouter Maes
Aug 5, 2017·Journal of Biomedical Optics·Wangzhong ShengAdah Almutairi
Dec 24, 2019·Frontiers in Bioengineering and Biotechnology·Xianhui LianQiang Ma
May 25, 2016·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Eva HemmerFiorenzo Vetrone
Aug 9, 2019·Materials·Srujan CherukuAnitha Ethirajan
Apr 8, 2021·Topics in Current Chemistry·Lei DongQing-Zheng Yang
Oct 16, 2020·Journal of Neural Engineering·Z FeketeA Zátonyi
Apr 8, 2019·ACS Biomaterials Science & Engineering·Yasmine BraekenAnitha Ethirajan
Dec 9, 2021·Topics in Current Chemistry·Jie ZhangDeman Han

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