Conjugates of synthetic cyclic peptides elicit bactericidal antibodies against a conformational epitope on a class 1 outer membrane protein of Neisseria meningitidis.

Infection and Immunity
P HoogerhoutC Peeters

Abstract

Bactericidal antibodies directed against surface loops of class 1 outer membrane proteins play a crucial role in protection against meningitis and sepsis caused by Neisseria meningitidis. So far, all efforts to obtain protective antibodies against these apparently conformational epitopes by using linear peptide analogs have been in vain. In this study, conjugates of head-to-tail cyclic peptides encompassing the predicted top of a protective surface loop were used for immunization. A series of 18 cyclic peptides with a ring size ranging from 7 to 17 residues, conjugated to tetanus toxoid, was investigated. Antipeptide and anti-whole-cell immunoglobulin G (IgG) titers elicited by the conjugates were determined. Conjugates of three peptides, containing 14, 15, and 17 amino acid residues (peptides 7, 12, and 13, respectively), induced an anti-whole-cell titer when Quillaja saponin A was used as the adjuvant. When alum was used as the adjuvant, the conjugate of peptide 12 did not elicit an anti-whole-cell response. From the Quillaja saponin A group, some of the sera obtained with conjugates of peptides 7 and 12 and all sera obtained with the peptide 13 conjugate were bactericidal in vitro. None of the sera evoked with alum as the ad...Continue Reading

References

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Citations

Mar 27, 1998·The Journal of Peptide Research : Official Journal of the American Peptide Society·K AugustynsG Jung
Nov 24, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·P HoogerhoutP J Roholl
Feb 27, 2015·Immunologic Research·Martiniano BelloJosé Correa-Basurto
Apr 9, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·G PatelD M Brennand
Oct 6, 2012·AIDS Research and Human Retroviruses·Nicos KarasavvasUNKNOWN MOPH TAVEG Collaboration
Feb 23, 2000·FEMS Immunology and Medical Microbiology·C JansenJ Tommassen
Jul 13, 2000·FEMS Immunology and Medical Microbiology·I R PeakM P Jennings
Mar 15, 2018·Nature Communications·S HollingsheadC M Tang
Oct 12, 2000·Biochemical and Biophysical Research Communications·G SardiñasR Silva
Feb 6, 2004·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Carmen ArigitaWim Jiskoot

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