Conjugating Methotrexate to magnetite (Fe(3)O(4)) nanoparticles via trichloro-s-triazine.

Journal of Materials Chemistry
Kaylie L YoungShouheng Sun

Abstract

Monodisperse Fe(3)O(4) nanoparticles (NPs) originally synthesized with a hydrophobic oleylamine capping ligand were made water soluble and conjugated to the anticancer drug Methotrexate (MTX) using a new chemistry based on the readily available linker trichloro-s-triazine (TsT). This new linker is much more versatile than those that currently exist for attaching biomolecules to magnetic NPs. The MTX-conjugated NPs were found to be stable under physiological conditions for over 72 hours and MTX was shown to maintain its anticancer activity after conjugation to the NP surface. Through cell viability studies and intracellular uptake studies, MTX-conjugated NPs were shown to have targeting specificity for a tumor cell line (9L rat glioma) over a healthy cell line (Cultured Pulmonary Artery Endothelial). Additionally the MTX-conjugated NPs were visualized inside 9L cells using fluorescence microscopy to help elucidate their path within a cell after internalization.

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Citations

Oct 31, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Min LiEn-Tang Kang
Nov 26, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Priyanka MurawalaB L V Prasad
Feb 11, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Rajendiran MangaiyarkarasiSingaravelu Ganesan
Jul 30, 2010·Journal of Materials Chemistry·Michael NickelsWellington Pham
Dec 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Beatriz Álvarez-GonzálezManuel Sánchez-Polo
May 16, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Bijaideep DuttaP A Hassan
Nov 16, 2016·Micron : the International Research and Review Journal for Microscopy·Maheshika P ArachchigeBhanu P Jena

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