Conjugation of an α-Helical Peptide to the Surface of Gold Nanoparticles

Langmuir : the ACS Journal of Surfaces and Colloids
Logan M WilderRichard M Crooks

Abstract

We are interested in functionalizing gold nanoparticles (AuNPs) with proteins using a biomimetic approach in which an intermediate peptide "glue" directs the orientation of a protein relative to the AuNP surface. The first step toward this goal is described in the present article. Specifically, we show that ∼5 nm AuNPs can be functionalized with a mixed self-assembled monolayer (SAM) consisting of oligo(ethylene glycol) alkanethiols terminated with either hydroxyl or azide groups, and that the resulting materials are stable and soluble in water. The azide groups on the surface of the AuNPs can be subsequently linked to alkyne-functionalized peptides via a copper-catalyzed azide-alkyne cycloaddition (click) reaction. Analysis of the resulting material by Fourier transform infrared and circular dichroism spectroscopy demonstrates that the peptide is covalently linked to the SAM and that it exists in an α-helical conformation. In addition to our intended purpose of using these highly structured, biomimetic materials to orient proteins, they may also be useful for applications involving interactions between nanoparticles and cells.

References

Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T K ChangJ H Richards
Mar 3, 1981·Biochemistry·J P Hennessey, W C Johnson
Feb 26, 2000·Trends in Pharmacological Sciences·M LindgrenU Langel
Jul 28, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Alexey A VertegelJonathan S Dordick
Aug 12, 2004·Journal of the American Chemical Society·Raphaël LévyDavid G Fernig
Sep 2, 2004·Journal of the American Chemical Society·Ayush VermaVincent M Rotello
Mar 23, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·P V BowerG P Drobny
Apr 14, 2005·Chemical Reviews·Nathaniel L Rosi, Chad A Mirkin
Jun 2, 2005·Journal of Colloid and Interface Science·Nobuyuki HigashiMasazo Niwa
Nov 1, 2005·Journal of Colloid and Interface Science·Michael J Read, Sandra L Burkett
Mar 23, 2006·Journal of the American Chemical Society·Paul RoachCarole C Perry
Sep 26, 2006·Colloids and Surfaces. B, Biointerfaces·Xiong LiuQun Huo
Apr 27, 2007·Journal of the American Chemical Society·Himadri S Mandal, Heinz-Bernhard Kraatz
Jan 31, 2008·Chemical Reviews·Matthew E StewartRalph G Nuzzo
May 27, 2008·Nature Materials·Ayush VermaFrancesco Stellacci
Aug 12, 2008·Biomedical Materials·M-E Aubin-Tam, K Hamad-Schifferli
Aug 14, 2008·Chemical Reviews·Morten Meldal, Christian Wenzel Tornøe
Feb 10, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Scott M RosendahlIan J Burgess
Feb 18, 2009·Bioconjugate Chemistry·Zeljka KrpetićMathias Brust
Feb 26, 2010·Angewandte Chemie·Chun-Long Chen, Nathaniel L Rosi
Jul 31, 2010·Chemical Society Reviews·Joseph M Slocik, Rajesh R Naik
Nov 3, 2010·Current Opinion in Chemical Biology·Subinoy RanaVincent M Rotello
Dec 1, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Alison M W Reed, Steven J Metallo
Apr 12, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Daniel F Moyano, Vincent M Rotello
Jun 9, 2011·Chemical Reviews·Matthew R JonesChad A Mirkin
Oct 4, 2011·Biotechnology Advances·Shakeel Ahmed Ansari, Qayyum Husain
Jan 20, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Ignacio F Gallardo, Lauren J Webb
Feb 3, 2012·Chemical Reviews·Krishnendu SahaVincent M Rotello
Jan 21, 2014·Bioconjugate Chemistry·Seoung-Ryoung ChoiC Dale Poulter
Oct 4, 2014·Science·Philip D HowesMolly M Stevens
Jul 3, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Jason W Dugger, Lauren J Webb
Oct 16, 2015·Bioconjugate Chemistry·Rodrigo D VinluanJie Zheng
Mar 7, 2008·Sensors·Dorothee GrieshaberErik Reimhult

❮ Previous
Next ❯

Citations

Apr 15, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Cristina De La Encarnacion BermudezDamiano Genovese
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaotong LiZhenxin Wang
Dec 18, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Whitney A FiesLauren J Webb
Sep 18, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Raffaella BucciSara Pellegrino
Dec 9, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Julie Ann LebitaniaHitoshi Asakawa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.