PMID: 6988011Mar 14, 1980Paper

Conjugation of enzymes to immunoglobulins using dimaleimides

Biochimica Et Biophysica Acta
P D WestonR Wrigglesworth

Abstract

A method is described for coupling enzymes to immunoglobulins using sulphydryl derivatives of the proteins and a dimaleimide which is relatively water-soluble. Parameters affecting the performance of the conjugates have been examined including level of sulphydryl incorporation, ratio of enzyme/immunoglobulin and nature of dimaleimide used. Peroxidase-immunoglobulin conjugates made by the dimaleimide method have been compared with those made by the periodate oxidation method and found to have a superior performance. Immunoglobulin has been linked to peroxidase (horseradish peroxidase, EC 1.11.1.7), glucose oxidase from Aspergillus niger, (EC 1.1.3.4), penicillinase from Bacillus cereus beta-lactamase I (EC 3.5.2.6), and beta-galactosidase from Escherichia coli (EC 3.2.1.23).

References

Jan 1, 1978·Journal of Immunological Methods·E S RectorA H Sehon
Nov 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·A H Schuurs, B K Van Weemen
Jul 1, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·J H KennedyP Wilding
Jun 30, 1975·Annals of the New York Academy of Sciences·E J RuitenbergJ Buys
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane, A Kawaoi
Mar 1, 1967·Archives of Biochemistry and Biophysics·D R Grassetti, J F Murray
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May 1, 1962·The Biochemical Journal·R P NOVICK

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Citations

Nov 1, 1995·Thrombosis Research·F ManningB Woodhams
Feb 19, 2004·Advanced Drug Delivery Reviews·Carsten EhrhardtUdo Bakowsky
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·D D SpraggM A Gimbrone
Jan 18, 2006·Acta Pharmacologica Sinica·Jun LiZhao-lai Chen
May 8, 1990·Biochimica Et Biophysica Acta·C M LinR J Krueger

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