PMID: 9172827Jan 1, 1997Paper

Conjugation-specific genes in the ciliate Euplotes crassus: gene expression from the old macronucleus

The Journal of Eukaryotic Microbiology
Z LingL A Klobutcher

Abstract

Following mating or conjugation, the hypotrichous ciliate Euplotes crassus undergoes a massive genome reorganization process. While the nature of the rearrangement events has been well studied, little is known concerning proteins that carry out such processes. As a means of identifying such proteins, differential screening of a developmental cDNA library, as well as construction of a cDNA subtraction library, was used to isolate genes expressed only during sexual reproduction. Five different conjugation-specific genes have been identified that are maximally expressed early in conjugation, during the period of micronuclear meiosis, which is just prior to macronuclear development and the DNA rearrangement process. All five genes are retained in the mature macronucleus. Micronuclear, macronuclear, and cDNA clones of one gene (conZA7) have been sequenced, and the results indicate that the gene encodes a putative DNA binding protein. In addition, the presence of an internal eliminated sequence in the micronuclear copy of the conZA7 gene indicates that this conjugation-specific gene is transcribed from the old macronucleus.

References

Oct 1, 1991·Current Opinion in Genetics & Development·L A Klobutcher, C L Jahn
Mar 1, 1991·The Journal of protozoology·S E Baird, L A Klobutcher
Nov 1, 1985·The Journal of protozoology·D W MartindaleP J Bruns
Feb 11, 1986·Nucleic Acids Research·D W MartindaleP J Bruns
Mar 25, 1988·Nucleic Acids Research·D W Martindale, F M Taylor
Apr 10, 1987·Cell·G R Fink
Feb 1, 1968·Experimental Cell Research·M V Rao
Mar 4, 1983·Science·R Lewin
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R E BoswellD M Prescott
Mar 25, 1981·Journal of Molecular Biology·T F Smith, M S Waterman
Apr 1, 1995·Current Opinion in Genetics & Development·S J Triezenberg
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·L A Klobutcher
May 1, 1994·The Journal of Eukaryotic Microbiology·C M PriceJ R Vermeesch
Jan 1, 1994·Molecular and Cellular Biology·J R Vermeesch, C M Price
Jan 1, 1993·Genes & Development·J W Jaraczewski, C L Jahn
Jan 1, 1996·Trends in Genetics : TIG·M C Yao
Nov 1, 1996·The Journal of Eukaryotic Microbiology·M E Jacobs, L A Klobutcher

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Citations

Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·C L JahnL A Klobutcher
Nov 30, 2000·Nucleic Acids Research·D M Prescott, S J Dizick
Feb 13, 2003·Eukaryotic Cell·Mary Ellen JacobsLawrence A Klobutcher
Feb 20, 1998·Current Opinion in Genetics & Development·D M Prescott
May 28, 2013·European Journal of Protistology·Phyllis Clarke BradburyLimin Zhang
Mar 21, 2000·Genesis : the Journal of Genetics and Development·S Ghosh, L A Klobutcher
Apr 6, 2000·The Journal of Eukaryotic Microbiology·M E JacobsL A Klobutcher

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