Conjugative plasmids and the degradation of arylsulfonates in Comamonas testosteroni.

Applied and Environmental Microbiology
F Junker, A M Cook

Abstract

Comamonas testosteroni T-2 degrades p-toluenesulfonate (TSA) via p-sulfobenzoate (PSB) and protocatechuate and degrades toluenecarboxylate via terephthalate (TER) and protocatechuate. The appropriate genes are expressed in at least five regulatory units, some of which are also found in C. testosteroni PSB-4 (F. Junker, R. Kiewitz, and A. M. Cook, J. Bacteriol. 179:919-927, 1997). C. testosteroni T-2 was found to contain two plasmids, pTSA (85 kbp) and pT2T (50 kbp); a TSA- mutant (strain TER-1) contained only plasmid pT2T. C. testosteroni PSB-4, which does not degrade TSA, contained one plasmid, pPSB (85 kbp). The type strain contained no plasmids. Conjugation experiments showed that plasmid pTSA (possibly in conjunction with pT2T) was conjugative, and the single copy of the TSA operon (tsaMBCD) with its putative regulator gene (tsaR) in strain T-2 was found on plasmid pTSA, which also carried the PSB genes (psbAC) and presumably transport for both substrates. Plasmid pTSA was assigned to the IncP1 beta group and was found to carry two copies of insertion element IS1071. Plasmid pPSB (of strain PSB-4), which could be maintained in strains with plasmid pTSA or pT2T, was also conjugative and was found to carry the PSB genes as we...Continue Reading

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Citations

Nov 29, 2005·Environmental Microbiology·Dick B JanssenPeter Terpstra
Feb 3, 2006·Biodegradation·Raffaella BenedettiGiuseppe Firrao
Dec 20, 2015·Applied and Environmental Microbiology·Kai ChenJiandong Jiang
Jan 5, 2006·Applied and Environmental Microbiology·Masahiro SotaMasataka Tsuda
May 9, 1998·Applied and Environmental Microbiology·D Di GioiaR C Wyndham
Oct 20, 2005·Journal of Bioscience and Bioengineering·M TsudaK Furukawa
Jun 25, 2015·Microbiology Spectrum·Ana SeguraJuan Luis Ramos

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