Conjunctival transcriptome profiling of Solomon Islanders with active trachoma in the absence of Chlamydia trachomatis infection.

Parasites & Vectors
Hristina VasilevaChrissy H Roberts

Abstract

Clinical signs of active (inflammatory) trachoma are found in many children in the Solomon Islands, but the majority of these individuals have no serological evidence of previous infection with Chlamydia trachomatis. In Temotu and Rennell and Bellona provinces, ocular infections with C. trachomatis were seldom detected among children with active trachoma; a similar lack of association was seen between active trachoma and other common bacterial and viral causes of follicular conjunctivitis. Here, we set out to characterise patterns of gene expression at the conjunctivae of children in these provinces with and without clinical signs of trachomatous inflammation-follicular (TF) and C. trachomatis infection. Purified RNA from children with and without active trachoma was run on Affymetrix GeneChip Human Transcriptome Array 2.0 microarrays. Profiles were compared between individuals with ocular C. trachomatis infection and TF (group DI; n = 6), individuals with TF but no C. trachomatis infection (group D; n = 7), and individuals without TF or C. trachomatis infection (group N; n = 7). Differential gene expression and gene set enrichment for pathway membership were assessed. Conjunctival gene expression profiles were more similar wit...Continue Reading

References

Oct 17, 1998·Eye·A M Abu el-AsrarV Desmet
Feb 29, 2000·Epidemiology and Infection·R BaileyD Mabey
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jan 30, 2009·Bioinformatics·Davis J McCarthy, Gordon K Smyth
Aug 7, 2010·Bioinformatics·Benilton S Carvalho, Rafael A Irizarry
Nov 1, 2010·Cornea·Nobuyuki EbiharaAkira Murakami
Nov 8, 2012·PLoS Neglected Tropical Diseases·E Brook GoodhewPatrick J Lammie
Mar 5, 2013·PLoS Neglected Tropical Diseases·Victor H HuMatthew J Burton
Mar 19, 2013·Italian Journal of Pediatrics·Mario La RosaAlfredo Reibaldi
May 3, 2013·Journal of Clinical Microbiology·Chrissy H RobertsMartin J Holland
Aug 12, 2014·The Lancet Global Health·Rupert R A BourneUNKNOWN Vision Loss Expert Group
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
Jul 15, 2015·Ophthalmic Epidemiology·Anthony W SolomonAllen Foster
Jul 13, 2016·PLoS Neglected Tropical Diseases·Colin K MacleodAnthony W Solomon
Dec 3, 2016·Nucleic Acids Research·Minoru KanehisaKanae Morishima
Sep 13, 2017·PLoS Neglected Tropical Diseases·Anaseini CamaUNKNOWN Global Trachoma Mapping Project

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Assay
MDA

Software Mentioned

R
Database for Annotation , Visualization and Integrated Discove...
Bioconductor package “
MASS
limma ”
Bioconductor
oligo ”

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.