Dec 10, 2013

Iodine staining as a useful probe for distinguishing amyloid fibril polymorphs

BioRxiv : the Preprint Server for Biology
Adam H MarblestoneGeorge M Church


Amyloid fibrils are protein aggregates with needle-like morphology and cross-β structure that are associated with amyloidoses and neurodegenerative diseases. It is recently suggested that amyloid polymorphism, i.e., structural diversity of amyloid fibrils, has a deep relationship with pathology, attracting a great deal of attention for understanding molecular mechanism of amyloid diseases. However, its prompt recognition is almost halted due to inadequate development of analytical methods for detecting polymorphism of amyloid fibrils sensitively and quickly. Here, we propose that iodine staining, a coloring reaction that occurs by complexing iodine with host compounds to form polyiodide ions, can be used as a method for distinguishing polymorphs of amyloid fibrils. When three polymorphs of insulin fibrils, NaCl fibrils, SDS fibrils, and no-salt fibrils, were prepared and iodine-stained, they exhibited different colors. Furthermore, each of these colors was inherited to daughter fibrils by seeding reactions. The colors were fundamentally represented as a sum of three absorption bands in visible region between 400-750 nm, and these bands showed different titration curves against iodine, suggesting that there are more than one spe...Continue Reading

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