Connecting proline and γ-aminobutyric acid in stressed plants through non-enzymatic reactions

PloS One
Santiago SignorelliJorge Monza

Abstract

The accumulation of proline (Pro) in plants exposed to biotic/abiotic stress is a well-documented and conserved response in most vegetal species. Stress conditions induce the overproduction of reactive oxygen species which can lead to cellular damage. In vitro assays have shown that enzyme inactivation by hydroxyl radicals (·OH) can be avoided in presence of Pro, suggesting that this amino acid could act as an ·OH scavenger. We applied Density Functional Theory coupled with a polarizable continuum model to elucidate how Pro reacts with ·OH. In this work we suggest that Pro reacts favourably with ·OH by H-abstraction on the amine group. This reaction produces the spontaneous decarboxylation of Pro leading to the formation of pyrrolidin-1-yl. In turn, pyrrolidin-1-yl can easily be converted to Δ1-pyrroline, the substrate of the enzyme Δ1-pyrroline dehydrogenase, which produces γ-aminobutyric acid (GABA). GABA and Pro are frequently accumulated in stressed plants and several protective roles have been assigned to these molecules. Thereby we present an alternative non-enzymatic way to synthetize GABA under oxidative stress. Finally this work sheds light on a new beneficial role of Pro accumulation in the maintenance of photosynthet...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·B Halliwell, J M Gutteridge
Jan 1, 1985·Molecular Aspects of Medicine·B Halliwell, J M Gutteridge
Apr 1, 1972·Journal of Bacteriology·S Dover, Y S Halpern
Apr 1, 1984·The Biochemical Journal·B Halliwell, J M Gutteridge
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Apr 1, 1997·International Journal of Biological Macromolecules·S MiertusE Chiellini
Oct 26, 1999·Trends in Plant Science·B J ShelpM D McLean
Jul 13, 2000·Cellular and Molecular Life Sciences : CMLS·J DatF Van Breusegem
Jul 25, 2000·Advances in Microbial Physiology·P Poole, D Allaway
Oct 23, 2001·Amino Acids· AliaJ Matysik
Aug 17, 2002·Current Opinion in Plant Biology·Steven NeillJohn Hancock
Sep 18, 2002·Trends in Plant Science·Ron Mittler
Dec 21, 2002·The Plant Journal : for Cell and Molecular Biology·L J SweetloveA H Millar
Oct 1, 1961·Journal of General Microbiology·Y S HALPERN, H E UMBARGER
Mar 9, 2004·Trends in Plant Science·Nicolas Bouché, Hillel Fromm
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Changbin Chen, Martin B Dickman
Feb 16, 2006·Journal of the American Chemical Society·Josef KapitánPetr Bour
Sep 1, 1966·Plant Physiology·N M Barnett, A W Naylor
Jul 13, 2006·The Journal of Physical Chemistry. a·Hrant P HratchianH Bernhard Schlegel
Oct 27, 2006·Journal of Computational Chemistry·I Mayer
Dec 25, 2007·Trends in Plant Science·Aaron FaitAlisdair R Fernie
Jan 12, 2008·Accounts of Chemical Research·Yan Zhao, Donald G Truhlar
Dec 29, 2009·Trends in Plant Science·László Szabados, Arnould Savouré
Mar 23, 2010·Journal of Chemical Theory and Computation·Steven E Wheeler, K N Houk
Nov 5, 2010·Journal of the American Chemical Society·Steve Scheiner, Tapas Kar
Jul 17, 2012·Plant Science : an International Journal of Experimental Plant Biology·Barry J ShelpCarolyne J Brikis
Feb 7, 2013·Plant Physiology and Biochemistry : PPB·Santiago SignorelliJorge Monza

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Citations

Apr 22, 2015·The Plant Journal : for Cell and Molecular Biology·Andrea MatrosWim Van den Ende
Sep 1, 2015·Frontiers in Plant Science·Mariko Takayama, Hiroshi Ezura
Jul 1, 2015·Journal of Plant Physiology·Cirenia Arias-BaldrichSofía García-Mauriño
Jun 25, 2015·Frontiers in Plant Science·Simon Michaeli, Hillel Fromm
Feb 20, 2018·Frontiers in Plant Science·Petronia Carillo
Jan 31, 2020·Plant, Cell & Environment·Łukasz P TarkowskiMonica Höfte
May 8, 2020·Plant Signaling & Behavior·Ramakrishna Akula, Soumya Mukherjee
Aug 15, 2020·Frontiers in Plant Science·Ahmed El MoukhtariArnould Savouré
Sep 17, 2020·Organic & Biomolecular Chemistry·Joses G NathanaelUta Wille
Aug 22, 2017·Frontiers in Microbiology·Giuseppe ForlaniAndrzej Joachimiak
Feb 11, 2019·Plant Cell Reports·Maryam SeifikalhorOksana Lastochkina
Sep 9, 2019·Archives of Biochemistry and Biophysics·Stefanie BrandsErwin A Galinski
Apr 7, 2020·Journal of Environmental Management·Rafał SzewczykPrzemysław Bernat
Jan 10, 2018·Journal of Agricultural and Food Chemistry·Jeongeun LeeHiroshi Ezura
Jul 27, 2018·New Biotechnology·Kateřina PodlešákováNuria De Diego

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Methods Mentioned

BETA
transgenic

Software Mentioned

PCM
IEF
Gaussview
VMD
Gaussian09

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