Connexin 26-mediated gap junctional intercellular communication suppresses paracellular permeability of human intestinal epithelial cell monolayers

Experimental Cell Research
Hidekazu MoritaYasuo Suzuki

Abstract

In some cell types, gap junctional intercellular communication (GJIC) is associated with tight junctions. The present study was performed to determine the roles of GJIC in regulation of the barrier function of tight junctions. Caco-2 human colonic cells were used as a monolayer model, and barrier function was monitored by measuring mannitol permeability and transepithelial electrical resistance (TER). The monolayers were chemically disrupted by treatment with oleic acid and taurocholic acid. Western blotting analyses were performed to evaluate the protein levels of connexins, which are components of gap junctional intercellular channels. Cx26 expression was detected in preconfluent Caco-2 cells, and its level increased gradually after the monolayer reached confluency. These results prompted us to examine whether overexpression of Cx26 affects barrier function. Monolayers of Caco-2 cells stably expressing Cx26 showed significantly lower mannitol permeability and higher TER than mock transfectants when the monolayers were chemically disrupted. The levels of claudin-4, an important component of tight junctions, were significantly increased in the stable Cx26 transfectant. These results suggest that Cx26-mediated GJIC may play a cr...Continue Reading

References

Jun 1, 1996·Carcinogenesis·H Yamasaki, C C Naus
Apr 29, 1998·Annual Review of Physiology·J L Madara
Aug 26, 1998·Scandinavian Journal of Gastroenterology·P NejdforsB Jeppsson
Nov 13, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·S Tsukita, M Furuse
Dec 3, 1999·Biochemical and Biophysical Research Communications·T KojimaD C Spray
Jun 16, 2000·Advanced Drug Delivery Reviews·H ClarkeJ M Mullin
Jun 16, 2000·Advanced Drug Delivery Reviews·H A Edens, C A Parkos
Jun 16, 2000·Advanced Drug Delivery Reviews·A M HopkinsA Nusrat
Dec 1, 2000·Scandinavian Journal of Gastroenterology·A VenkatramanS Murthy
Jun 18, 2002·Cell Communication & Adhesion·B N GiepmansW H Moolenaar
Jul 20, 2002·Molecular Membrane Biology·W Howard Evans, Patricia E M Martin
Sep 27, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·W Howard Evans, Patricia E M Martin

❮ Previous
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Citations

Feb 13, 2014·Gastrointestinal Endoscopy·Yoshihiro IsomuraKazuhiko Koike
Jun 15, 2007·The Journal of Membrane Biology·Takashi KojimaNorimasa Sawada
May 13, 2008·International Journal of Radiation Biology·Siobhan M MoyesKatharine E Carr
Mar 21, 2006·Journal of Cellular Physiology·Kunihiko NagasawaNorimasa Sawada
Dec 3, 2008·Biochimica Et Biophysica Acta·Mickaël DerangeonJean-Claude Hervé
Dec 19, 2007·Biochimica Et Biophysica Acta·Sigrid A RajasekaranAyyappan K Rajasekaran
Mar 6, 2013·Pharmacology & Therapeutics·Dana BazzounRabih Talhouk
Jun 19, 2015·Cellular and Molecular Life Sciences : CMLS·Michaël MaesMathieu Vinken
Jul 26, 2016·Frontiers in Neuroscience·Erica F DiezmosLu Liu
Jul 16, 2016·Scientific Reports·Sara Al-GhadbanMarwan E El-Sabban
Mar 19, 2014·FEBS Letters·Marijke De BockLuc Leybaert
May 23, 2007·Nutrition and Cancer·Chris I R GillIan Rowland
Sep 6, 2013·Microbiology and Molecular Biology Reviews : MMBR·Vanessa Liévin-Le Moal, Alain L Servin
May 10, 2005·International Journal of Cancer. Journal International Du Cancer·Chris I R GillIan Rowland
Oct 26, 2005·Journal of Cellular Physiology·Natacha TurckJean-François Launay
Sep 15, 2020·Journal of Gastroenterology and Hepatology·Keiji YokoyamaFumihito Hirai
May 7, 2015·World Journal of Gastroenterology : WJG·Takayoshi SuzukiTetsuya Mine
Jun 23, 2009·Journal of Cellular and Molecular Medicine·Gabriella AvielloFrancesca Borrelli
Feb 15, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·Shlok JindalGopinath Packirisamy
Jan 21, 2021·Biology·Laura Garcia-VegaPatricia E Martin
Aug 28, 2021·International Journal of Molecular Sciences·Iva SovadinováPavel Babica

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