Connexin 30 controls astroglial polarization during postnatal brain development

Development
Grégory GhézaliNathalie Rouach

Abstract

Astrocytes undergo intense morphological maturation during development, changing from individual sparsely branched cells to polarized and tremendously ramified cells. Connexin 30, an astroglial gap-junction channel-forming protein expressed postnatally, regulates in situ the extension and ramification of astroglial processes. However, the involvement of connexin 30 in astroglial polarization, which is known to control cell morphology, remains unexplored. We found that connexin 30, independently of gap-junction-mediated intercellular biochemical coupling, alters the orientation of astrocyte protrusion, centrosome and Golgi apparatus during polarized migration in an in vitro wound-healing assay. Connexin 30 sets the orientation of astroglial motile protrusions via modulation of the laminin/β1 integrin/Cdc42 polarity pathway. Connexin 30 indeed reduces laminin levels, inhibits the redistribution of the β1-integrin extracellular matrix receptors, and inhibits the recruitment and activation of the small Rho GTPase Cdc42 at the leading edge of migrating astrocytes. In vivo, connexin 30, the expression of which is developmentally regulated, also contributes to the establishment of hippocampal astrocyte polarity during postnatal matura...Continue Reading

References

Sep 8, 1992·The Journal of Comparative Neurology·D D HunterJ R Sanes
Apr 13, 2000·The European Journal of Neuroscience·P CalabresiA Gulino
Dec 20, 2002·Human Molecular Genetics·Barbara TeubnerKlaus Willecke
May 27, 2003·Journal of Cell Science·Kris A DeMali, Keith Burridge
May 28, 2003·Biochemical and Biophysical Research Communications·Martina BeltramelloPaola D'Andrea
Mar 5, 2004·Journal of Cell Science·Diane E Frank, William G Carter
Mar 24, 2006·The European Journal of Neuroscience·Nathalie RouachMartine Tencé
Oct 20, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Xunwei WuCord Brakebusch
Nov 4, 2006·Current Biology : CB·Naël OsmaniSandrine Etienne-Manneville
Dec 27, 2006·Molecular and Cellular Neurosciences·Christophe EglesDale D Hunter
May 18, 2007·Progress in Biophysics and Molecular Biology·Jean-Claude HervéHeather S Duffy
Aug 24, 2007·Nature·Laura A B EliasArnold R Kriegstein
Jun 19, 2008·Cellular Signalling·Soniya Sinha, Wannian Yang
Aug 1, 2008·The Journal of Biological Chemistry·Mickaël DerangeonJean-Claude Hervé
Oct 9, 2008·Current Opinion in Neurobiology·Sandrine Etienne-Manneville
Mar 17, 2009·Experimental Cell Research·Chunyan QuIris Schrijver
Aug 24, 2010·Nature Reviews. Molecular Cell Biology·J Thomas ParsonsMartin A Schwartz
Dec 22, 2010·The Journal of Cell Biology·Naël OsmaniSandrine Etienne-Manneville
May 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ulrike PannaschNathalie Rouach
Jul 14, 2011·Journal of Cell Science·Steven MachtalerLinda Matsuuchi
Sep 6, 2012·Developmental Neurobiology·Huashan PengSalvatore Carbonetto
Jan 5, 2013·Brain Structure & Function·Lisiani SaurLéder Leal Xavier
Feb 1, 2014·Nature Protocols·Arezki BoudaoudOlivier Hamant
Mar 4, 2014·Nature Neuroscience·Ulrike PannaschNathalie Rouach
Sep 17, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Stefanie AndersChristian Henneberger
Jan 27, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Adeline BergerAlexis-Pierre Bemelmans

❮ Previous
Next ❯

Citations

Sep 1, 2019·Current Alzheimer Research·Laura Trujillo-EstradaFrank M LaFerla
Nov 8, 2018·Nature Reviews. Neuroscience·Glenn DalléracNathalie Rouach
Jun 13, 2019·International Journal of Molecular Sciences·Sara EitelmannJonathan Stephan
Jul 28, 2018·International Journal of Molecular Sciences·Raúl Lagos-CabréLisette Leyton
Dec 14, 2018·Frontiers in Cellular Neuroscience·Simon L WadleJonathan Stephan
Oct 11, 2020·International Journal of Molecular Sciences·Sara EitelmannJonathan Stephan
Aug 28, 2020·Reviews in the Neurosciences·Dorothea DereEkrem Dere
Mar 30, 2021·Frontiers in Cellular Neuroscience·Jonathan StephanMin Zhou
Jul 3, 2021·Science·Jérôme RibotNathalie Rouach

❮ Previous
Next ❯

Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
atomic force microscopy
pull-down
GTPase
GTPases
electrophoresis
scraping
protein assay
PCR

Software Mentioned

Analyze Particle
Clampfit10
Clampex10
ImageJ
FibrilTool

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.