Connexin 32 dominant-negative mutant transgenic rats are resistant to hepatic damage by chemicals

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Makoto AsamotoTomoyuki Shirai

Abstract

Connexins are subunits of gap junction channels, which allow direct transfer of ions, secondary messenger molecules, and other metabolites between contacting cells. Gap junctions are believed to be involved in tissue homeostasis, embryonic development, and control of cell proliferation. Several studies have shown that cell damage signals are transmitted through gap junctions when cells are irradiated or when cells bearing the herpes simplex virus-thymidine kinase (HSV-TK) gene are treated with ganciclovir. We established 2 lines of transgenic rats with a dominant-negative mutant of connexin 32 gene under control of the albumin promoter. In the livers of transgenic rats, membrane localization of normal endogenous connexin 32 protein is disturbed, and gap junction capacity measured by scrape dye-transfer assay in vivo is markedly decreased when compared with wild-type rats. The present investigation concerned susceptibility to the liver-toxic substances D-galactosamine and carbon tetrachloride. These toxicants induced massive liver cell death and elevated serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in the wild-type rats; however, much fewer liver cells were damaged and serum enzyme elevation w...Continue Reading

References

Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·G ShiotaE V Schmidt
Mar 1, 1991·Neuron·M V BennettJ C Sáez
Jan 1, 1989·Trends in Neurosciences·S C Guthrie, N B Gilula
Jul 1, 1986·The Journal of Cell Biology·D L Paul
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K S ZaretD L Weinstat
Feb 4, 1972·Nature·N B GilulaA Steinbach
Oct 1, 1981·Physiological Reviews·W R Loewenstein
Sep 28, 1981·Life Sciences·J L Farber
Dec 24, 1993·Science·J BergoffenK H Fischbeck
Feb 9, 1996·Cell·N M Kumar, N B Gilula
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·M MesnilH Yamasaki
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·E NellesK Willecke
Feb 27, 1998·Frontiers in Bioscience : a Journal and Virtual Library·J E Trosko, R J Ruch
Apr 7, 2000·Brain Research. Brain Research Reviews·A F Andrade-RozentalD C Spray
Jul 20, 2002·Molecular Membrane Biology·W Howard Evans, Patricia E M Martin
Aug 27, 2002·Radiation Protection Dosimetry·J B LittleH Nagasawa

❮ Previous
Next ❯

Citations

Jul 18, 2008·Histochemistry and Cell Biology·Takahiro GotowYasuo Uchiyama
Nov 23, 2013·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Isao IgarashiAtsushi Sanbuissho
Jan 8, 2016·Toxicology Mechanisms and Methods·Michaël MaesMathieu Vinken
Jun 6, 2007·Micron : the International Research and Review Journal for Microscopy·Beatriz Brandão Vaz-de-LimaGlaucia M Machado-Santelli
Dec 7, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mathieu VinkenVera Rogiers
Aug 22, 2006·Cancer Science·Kazunari TsujimuraTomoyuki Shirai
Jul 27, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marion Maurel, Jean Rosenbaum
Dec 23, 2014·Journal of Biochemical and Molecular Toxicology·Ren WangShangrong Li
Mar 21, 2012·Archives of Biochemistry and Biophysics·Rekha KarJean X Jiang
Jun 9, 2015·Translational Research : the Journal of Laboratory and Clinical Medicine·Michaël MaesMathieu Vinken
May 23, 2012·Journal of Hepatology·Mathieu Vinken
Jun 9, 2016·Toxicology Mechanisms and Methods·Bruno CogliatiMaria L Z Dagli
Dec 9, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Niek P van TilJurgen Seppen
Jun 5, 2014·Scientific Reports·Chieko SaitoYoshihide Tsujimoto
Oct 10, 2018·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Axelle CooremanMathieu Vinken
Jan 31, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ryan C KennedyC Anthony Hunt
Jul 29, 2009·Critical Reviews in Biochemistry and Molecular Biology·Mathieu VinkenVera Rogiers
Nov 20, 2016·Clinical and Experimental Pharmacology & Physiology·Taynã Cristina TiburcioBruno Cogliati
Apr 22, 2015·World Journal of Gastroenterology : WJG·Jing-Yao ZhangChang Liu
Jan 23, 2016·International Journal of Molecular Medicine·Woo-Jae ParkJoo-Won Park
Jan 19, 2017·International Journal of Molecular Medicine·Pui WongGary Tse
Feb 9, 2017·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Takehiko NohmiNaomi Toyoda-Hokaiwado
Mar 25, 2019·Experimental and Therapeutic Medicine·Nan TangBihua Lin
Dec 17, 2020·Biomolecules·Erin E Mulkearns-HubertJustin D Lathia
May 1, 2021·Biomedicines·Lydie CarreresHervé Lerat
May 7, 2021·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Aya Naiki-Ito

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.