Connexin 40 and ATP-dependent intercellular calcium wave in renal glomerular endothelial cells.

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Ildiko TomaJános Peti-Peterdi

Abstract

Endothelial intracellular calcium ([Ca(2+)](i)) plays an important role in the function of the juxtaglomerular vasculature. The present studies aimed to identify the existence and molecular elements of an endothelial calcium wave in cultured glomerular endothelial cells (GENC). GENCs on glass coverslips were loaded with Fluo-4/Fura red, and ratiometric [Ca(2+)](i) imaging was performed using fluorescence confocal microscopy. Mechanical stimulation of a single GENC caused a nine-fold increase in [Ca(2+)](i), which propagated from cell to cell throughout the monolayer (7.9 +/- 0.3 microm/s) in a regenerative manner (without decrement of amplitude, kinetics, and speed) over distances >400 microm. Inhibition of voltage-dependent calcium channels with nifedipine had no effect on the above parameters, but the removal of extracellular calcium reduced Delta[Ca(2+)](i) by 50%. Importantly, the gap junction uncoupler alpha-glycyrrhetinic acid or knockdown of connexin 40 (Cx40) by transfecting GENCs with Cx40 short interfering RNA (siRNA) almost completely eliminated Delta[Ca(2+)](i) and the calcium wave. Breakdown of extracellular ATP using a scavenger cocktail (apyrase and hexokinase) or nonselective inhibition of purinergic P2 receptor...Continue Reading

References

Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·M L CotrinaM Nedergaard
Aug 24, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M UrbanD C Spray
Jan 8, 2000·Microvascular Research·K Hwan Seul, E C Beyer
Jul 17, 2001·Histochemistry and Cell Biology·B ArensbakN H Holstein-Rathlou
Jan 16, 2002·The Journal of Biological Chemistry·Charles E StoutAndrew C Charles
Jul 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·V GreszS Nielsen
Jul 26, 2002·Journal of the American Society of Nephrology : JASN·Jian YaoTakashi Oite
Aug 9, 2002·American Journal of Physiology. Renal Physiology·Max SalomonssonNiels-Henrik Holstein-Rathlou
Dec 12, 2002·American Journal of Physiology. Renal Physiology·Frank SchwedaArmin Kurtz
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Phillip Darwin BellYasunobu Okada
Apr 26, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Kimiko YamamotoJoji Ando
May 17, 2003·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Lisa Ebihara
Aug 21, 2003·Physiological Genomics·Dumitru A IacobasDavid C Spray
Apr 20, 2004·Cardiovascular Research·Jacques-Antoine HaefligerPaolo Meda
May 20, 2004·Kidney International·Nese Akis, Michael P Madaio
Nov 24, 2004·Clinical and Experimental Pharmacology & Physiology·Nicole M Rummery, Caryl E Hill
Jan 27, 2005·Biochemical Pharmacology·Robson Coutinho-SilvaPhilip M Dunn
Apr 13, 2005·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Akira NishiyamaEdward W Inscho
Aug 4, 2005·American Journal of Physiology. Renal Physiology·Fiona McCullochJános Peti-Peterdi
Jan 7, 2006·Nature Medicine·Geoffrey Burnstock
Jan 28, 2006·The Journal of Clinical Investigation·Jacques-Antoine HaefligerPaolo Meda
Feb 24, 2006·American Journal of Physiology. Renal Physiology·János Peti-Peterdi
May 16, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hideo IchimuraJahar Bhattacharya
Jul 21, 2006·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·László Rosivall, János Peti-Peterdi
Jul 27, 2006·American Journal of Physiology. Renal Physiology·László RosivallJános Peti-Peterdi
Nov 14, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Torben R UhrenholtSteven S Segal
Dec 7, 2006·American Journal of Physiology. Renal Physiology·T R UhrenholtO Skøtt
Jan 11, 2007·American Journal of Physiology. Renal Physiology·Ian RiveraEdward W Inscho
Jan 27, 2007·Circulation Research·Charlotte WagnerFrank Schweda
Mar 3, 2007·Journal of the American Society of Nephrology : JASN·Lisa KurtzCharlotte Wagner
Jul 12, 2007·Kidney International·N KrattingerJ-A Haefliger
Jul 19, 2008·Neuron Glia Biology·Eliana ScemesDavid C Spray

❮ Previous
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Citations

Apr 28, 2012·Pflügers Archiv : European journal of physiology·Douglas K Atchison, William H Beierwaltes
Jun 30, 2012·Pflügers Archiv : European journal of physiology·Charlotte Wagner, Armin Kurtz
Mar 31, 2009·Purinergic Signalling·P Darwin BellZhi-Ren Zhang
Apr 25, 2012·Purinergic Signalling·Eduardo R Lazarowski
Jul 16, 2013·Progress in Neurobiology·Marijke De BockLuc Leybaert
Jun 9, 2012·Cardiovascular Research·Alexander W LohmanBrant E Isakson
Jun 27, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Fiona HannerJános Peti-Peterdi
Mar 26, 2010·American Journal of Physiology. Renal Physiology·David A Osmond, Edward W Inscho
Apr 2, 2011·American Journal of Physiology. Renal Physiology·J David HoltzclawSteven C Sansom
Jul 31, 2009·American Journal of Physiology. Renal Physiology·William H Beierwaltes
Oct 28, 2011·American Journal of Physiology. Renal Physiology·János Peti-PeterdiMatthias J Hackl
May 16, 2009·Journal of the American Society of Nephrology : JASN·Armin JustWilliam J Arendshorst
May 30, 2009·Journal of the American Society of Nephrology : JASN·Arnold SiposJános Peti-Peterdi
Nov 23, 2013·Purinergic Signalling·Geoffrey BurnstockMatthew A Bailey
Apr 10, 2014·The Journal of Clinical Investigation·James L BurfordJános Peti-Peterdi
May 31, 2012·Cell Communication & Adhesion·Nadia HalidiJean-Jacques Meister
Feb 10, 2016·Frontiers in Physiology·Mauricio A RetamalCarlos González
Dec 26, 2008·Kidney International·Frank SchwedaCharlotte Wagner
Oct 5, 2010·Cell Calcium·Lionel F Jaffe
Mar 7, 2012·Cell Biochemistry and Function·Anjana Chandrasekhar, Amal Kanti Bera
Nov 29, 2011·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Charlotte Mehlin Sorensen, Niels-Henrik Holstein-Rathlou
Jun 21, 2011·Biochimica Et Biophysica Acta·Armin Kurtz
Nov 4, 2016·Proceedings of the National Academy of Sciences of the United States of America·Federico CerianiFabio Mammano
Jan 27, 2017·BMC Cell Biology·Daniela BegandtXavier F Figueroa
Mar 31, 2018·International Journal of Molecular Sciences·J Matthew Rhett, Elizabeth S Yeh
May 28, 2011·Journal of the American Society of Nephrology : JASN·Carie S Facemire, Susan B Gurley
Dec 17, 2008·American Journal of Physiology. Renal Physiology·Jian YaoMasanori Kitamura
Apr 3, 2015·Physiological Reviews·Mattias CarlströmWilliam J Arendshorst
Aug 23, 2019·Physiological Reviews·Volker VallonBellamkonda K Kishore
Apr 15, 2009·Physiology·János Peti-PeterdiSarah L Vargas
Jul 20, 2012·Physiological Reviews·Luc Leybaert, Michael J Sanderson
Oct 20, 2018·Journal of Cell Science·Nicholas MikolajewiczSvetlana V Komarova
Feb 21, 2014·American Journal of Physiology. Renal Physiology·Hila Roshanravan, Stuart E Dryer
Jan 9, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Damiano BurattoFabio Mammano

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