Connexin 43 Affects Osteogenic Differentiation of the Posterior Longitudinal Ligament Cells via Regulation of ERK Activity by Stabilizing Runx2 in Ossification

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Haisong YangJiangang Shi

Abstract

Connexin 43 is one of the most potent gap junction proteins related to osteoblast differentiation and bone formation. We hypothesized that Connexin 43 is a significant factor in osteogenic differentiation in the posterior longitudinal ligament through the regulation of extracellular signal-regulated kinases (ERK) activity by converging on Runt-related transcription factor 2 (Runx2) activity. In this study, we mapped the activity of Connexin 43 to ERK and Runx2 by extracting longitudinal ligament cell for culture and silencing Connexin expression in addition to dexamethasone treatment in vitro. qRT-PCR, Western Blot, and Runx2-responsive Luciferase Reporter Assay were performed to detect the activity of ERK, Runx2 and the expression levels of osseous genes under Connexin 43 modification. Downregulation of Connexin 43 resulted in suppression of dexamethasone-induced osteogenic differentiation, inhibition of the ERK and Runx2 activity, and reduction of osseous gene expression. these data support that Connexin 43 significantly regulates osteogenic differentiation in the cells from posterior longitudinal ligament by altering the activity of ERK, and subsequently causing the modification of Runx2.

Citations

Dec 23, 2017·Cell Biology International·Xiaoting FanWen-Song Tan
Mar 30, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tao WangMing H Zheng
Jul 12, 2019·Journal of Orthopaedic Surgery and Research·Peng WangShibao Lu
Mar 14, 2019·International Journal of Molecular Medicine·Peng WangShibao Lu
Apr 19, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Atum M BuoJoseph P Stains

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