Connexin 43 and Its Hemichannels Mediate Hypoxia-Ischemia-Induced Cell Death in Neonatal Rats

Child Neurology Open
Jingwei WangBojun Zhao

Abstract

Wistar rat pups had the left common carotid artery cut, and they were exposed to 8% oxygen with free access to food and water until they were killed at 1, 12, 24, and 48 hours after the hypoxia-ischemia (HI) insult. Connexin 43 (Cx43), hemichannel (HC1), and caspase 3 (Casp3) in cerebral HI tissues were examined by immunohistochemistry and Western blot analyses. Astrocytes cell line, astrocytes transduced with a retroviral empty vector (Psup astrocyte), or a Cx43-specific small hairpin RNA (shRNA) construct (shRNA astrocytes) was treated with oxygen-glucose deprivation (OGD) insult. The viability of astrocytes was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The results showed the expression of Cx43, HC1, and Casp3 in rats' brain, and astrocytes and Psup astrocytes increased significantly after 24 hours of HI/OGD insult. Cell viability decreased after 24 hours of the insult. The results suggest that Cx43 and hemichannel are likely to mediate the astrocytic death after HI insult.

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Citations

Jul 28, 2018·Scientific Reports·Frazer P CoutinhoIlva D Rupenthal
Mar 16, 2019·Frontiers in Physiology·Kelly Q ZhouJoanne O Davidson
Apr 23, 2020·Drug Delivery and Translational Research·Frazer P CoutinhoIlva D Rupenthal
Apr 4, 2021·International Journal of Molecular Sciences·Jorge González-CasanovaDiana Rojas-Gomez

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Methods Mentioned

BETA
electrophoresis
protein assay
Transfection

Software Mentioned

ImageJ
GraphPad Prism
Prism
SCION IMAGE

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