Connexin 43 expressed in endothelial cells modulates monocyte‑endothelial adhesion by regulating cell adhesion proteins

Molecular Medicine Reports
Dongdong YuanZiqing Hei

Abstract

Adhesion between circulating monocytes and vascular endothelial cells is a key initiator of atherosclerosis. In our previous studies, it was demonstrated that the expression of connexin (Cx)43 in monocytes modulates cell adhesion, however, the effects of the expression of Cx43 in endothelial cells remains to be elucidated. Therefore, the present study investigated the role of the expression of Cx43 in endothelial cells in the process of cell adhesion. A total of four different methods with distinct mechanisms were used to change the function and expression of Cx43 channels in human umbilical vein endothelial cells: Cx43 channel inhibitor (oleamide), enhancer (retinoic acid), overexpression of Cx43 by transfection with pcDNA‑Cx43 and knock‑down of the expression of Cx43 by small interfering RNA against Cx43. The results indicated that the upregulation of the expression of Cx43 enhanced monocyte‑endothelial adhesion and this was markedly decreased by downregulation of Cx43. This mechanism was associated with Cx43‑induced expression of vascular cell adhesion molecule‑1 and intercellular cell adhesion molecule‑1. The effects of Cx43 in endothelial cells was independent of Cx37 or Cx40. These experiments suggested that local regulat...Continue Reading

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Citations

Jun 22, 2017·Neurochemical Research·Marijke De BockChristian Giaume
Jan 30, 2020·International Journal of Molecular Sciences·Abhishek K Singh, Jose A Cancelas
Jun 8, 2018·International Journal of Molecular Sciences·Filippo MolicaJonathan M Gibbins
Feb 23, 2019·Frontiers in Pharmacology·Shuxian HanFulong Liao
Sep 24, 2019·Experimental Mechanics·M M Islam, R L Steward
Sep 25, 2019·International Journal of Molecular Sciences·Francisca HofmannFlavio Salazar-Onfray
Jan 4, 2021·Cellular and Molecular Life Sciences : CMLS·Raghda RamadanLuc Leybaert
Nov 5, 2021·BMC Anesthesiology·Dongdong YuanDezhao Liu

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