Abstract
A communication-deficient cell line (RIN cells, derived from a rat islet tumour), stably transfected with cDNA coding for rat connexin43 (Cx43), was chosen to further assess the mechanism of voltage gating of Cx43 gap junction channels. The experiments were carried out on preformed cell pairs using a dual whole-cell, voltage-clamp method. The junctional current, Ij, revealed a time- and voltage-dependent inactivation at transjunctional voltages Vj>+/-40 mV. When an asymmetrical pulse protocol was used (in cell 1 the holding potential was maintained, in cell 2 it was altered to establish a variable Vj), the channels exhibited an asymmetrical gating behaviour: Vj,0=-73.7 mV and 65.1 mV for negative and positive Vj, respectively (Vj at which Ij is half-maximally inactivated); gj(min)=0.34 and 0.29 (normalized minimal conductance); tau = 350 ms and 80 ms at Vj=100 mV (time constant of Ij inactivation). Hence, these parameters were more sensitive to positive Vj values. When a symmetrical pulse protocol was used (the holding potentials in cell 1 and cell 2 were altered simultaneously in steps of equal amplitude but of opposite polarity), the Vj -dependent asymmetries were absent: Vj,0=-60.5 and 59.5; gj (min)=0.27 and 0.29; tau =64 m...Continue Reading
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