Consequences of cadmium toxicity in rat hepatocytes: mitochondrial dysfunction and lipid peroxidation

Toxicology
L Muller

Abstract

Cadmium (Cd) (10-100 microM) decreased the ATP/ADP ratio and enhanced lipid peroxidation (LPO) (measured as thiobarbituric acid reactants) in incubated rat hepatocytes. Analysis of the subcellular distribution of Cd indicated its preferential attachment to the inner membranes of mitochondria. Incubation of isolated mitochondria with 0.005-0.05 microM Cd resulted in increased formation of formazans from nitroblue tetrazolium salts, indicating enhanced membrane permeability to succinate. These Cd-concentrations also diminished mitochondrial ATP. LPO in mitochondria strongly increased only after Cd-exposures above 1 microM Cd. Similarly, in Cd-treated hepatocytes decreases in ATP/ADP ratios corresponded to increases in LPO stimulation only at 30 and 60 min but not at 15 min of incubation when ATP/ADP ratios were already affected. Moreover, neither hepatocellular ATP/ADP decrease nor mitochondrial formazan formation due to Cd were prevented by (+)-cyanidanol-3, an effective inhibitor of Cd-induced LPO. These data suggest that even low Cd-concentrations in the hepatocyte disturb the integrity of its mitochondrial membranes concomitantly impairing the hepatocellular energy supply. LPO, only observed at higher Cd-concentrations, is no...Continue Reading

References

Apr 1, 1979·Journal of Inorganic Biochemistry·D SolaimanD H Petering
Apr 1, 1982·Biochemical Pharmacology·M YamaguchiS Okada
May 1, 1980·Toxicology and Applied Pharmacology·N H StaceyC D Klaassen
Jan 1, 1981·Journal of Toxicology and Environmental Health·N H Stacey, C D Klaassen
Jun 1, 1984·Toxicology·L Müller, F K Ohnesorge
Feb 1, 1982·Journal of Toxicology and Environmental Health·N H Stacey, H Kappus
Jul 1, 1982·Journal of Toxicology and Environmental Health·K S SantoneJ V Bruckner
Jun 1, 1980·Environmental Research·J O JärvisaloN E Saris

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Citations

Jan 12, 2000·Journal of Biochemical and Molecular Toxicology·L E Rikans, T Yamano
Sep 5, 2002·Journal of Applied Toxicology : JAT·Vilena KasubaMaja Blanusa
Feb 24, 2001·Archives of Biochemistry and Biophysics·E CasalinoC Landriscina
Mar 1, 1988·Archives of Environmental Contamination and Toxicology·L MullerN H Stacey
Apr 1, 1988·Bulletin of Environmental Contamination and Toxicology·A K Agarwal
Feb 1, 1988·Bulletin of Environmental Contamination and Toxicology·L MullerN H Stacey
Jan 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J LiuC D Klaassen
Jul 1, 1989·Biological Trace Element Research·L Müller, N H Stacey
Jun 30, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Pham T N DiepCatherine Jumarie
Oct 6, 2007·Biological Trace Element Research·Haki KaraMahmut Yilmaz
Nov 1, 1987·Chemistry and Physics of Lipids·H Kappus
Dec 1, 1992·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A Hudecová, E Ginter
May 1, 1987·Toxicology·L Müller, M Wilhelm
Sep 18, 1995·Toxicology·L BucioM C Gutiérrez-Ruiz
Jan 1, 1989·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F Díaz-BarrigaJ L Saborío
Feb 1, 1995·Free Radical Biology & Medicine·S J Stohs, D Bagchi
Jun 7, 2002·Toxicology·Eric B Harstad, Curtis D Klaassen
Sep 3, 2002·Toxicology·Elisabetta CasalinoClemente Landriscina
Oct 29, 2003·Toxicology·Michael WaisbergDetmar Beyersmann
Dec 29, 1998·Toxicology·D YildizD W Armstrong
Jun 7, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·O J MesnaR A Andersen
Jun 5, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D RomeroA J García-Fernández

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