Consequences of cps mutation of Klebsiella pneumoniae on 1,3-propanediol fermentation

Applied Microbiology and Biotechnology
Ni-Ni GuoDe-Hua Liu

Abstract

The filtration in 1,3-propanediol (1,3-PD) downstream process is influenced by the large amounts of capsular polysaccharides (CPS) produced by Klebsiella pneumoniae CGMCC 1.6366. The morphological and fermentation properties were investigated with the CPS-deficient mutant K. pneumoniae CGMCC 1.6366 CPS. Similar biomass was obtained with CGMCC 1.6366, and the mutant strain in batch cultures indicating the cell growth was slightly inhibited by CPS defection. The viscosity of fermentation broth by mutant strain decreased by 27.45%. The flux with ceramic membrane filter was enhanced from 168.12 to 303.6 l h(-1) m(-2), exhibiting the great importance for downstream processing of 1,3-PD fermentation. The products spectrum of mutant isolate changed remarkably regarding to the concentration of fermentation products. The synthesis of important 1,3-PD and 2,3-butanediol was enhanced from 9.73 and 4.06 g l(-1) to 10.37 and 4.77 g l(-1) in batch cultures. The noncapsuled K. pneumoniae provided higher 1,3-PD yield of 0.54 mol mol(-1) than that of encapsuled wild parent in batch cultures. The fed-batch fermentation of mutant strain resulted in 1,3-PD concentration, yield, and productivity of 78.13 g l(-1), 0.53 mol mol(-1), and 1.95 g l(-1) ...Continue Reading

References

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Citations

May 22, 2013·Biotechnology Letters·Anu Jose MattamSyed Shams Yazdani
Feb 7, 2012·Bioprocess and Biosystems Engineering·Jung-Hee ChoDoyoung Seung
Mar 21, 2012·Journal of Industrial Microbiology & Biotechnology·Dong WeiJian Hao
Feb 13, 2018·Biotechnology Letters·Dong WeiJian Hao
Nov 23, 2017·Microbial Cell Factories·Xinjun FengGuang Zhao

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