Consequences of neurite transection in vitro.

Journal of Neurotrauma
N CengizE K Oğuz

Abstract

In order to quantify degenerative and regenerative changes and analyze the contribution of multiple factors to the outcome after neurite transection, we cultured adult mouse dorsal root ganglion neurons, and with a precise laser beam, we transected the nerve fibers they extended. Cell preparations were continuously visualized for 24 h with time-lapse microscopy. More distal cuts caused a more elongated field of degeneration, while thicker neurites degenerated faster than thinner ones. Transected neurites degenerated more if the uncut neurites of the same neuron simultaneously degenerated. If any of these uncut processes regenerated, the transected neurites underwent less degeneration. Regeneration of neurites was limited to distal cuts. Unipolar neurons had shorter regeneration than multipolar ones. Branching slowed the regenerative process, while simultaneous degeneration of uncut neurites increased it. Proximal lesions, small neuronal size, and extensive and rapid neurite degeneration were predictive of death of an injured neuron, which typically displayed necrotic rather than apoptotic form. In conclusion, this in vitro model proved useful in unmasking many new aspects and correlates of mechanically-induced neurite injury.

References

Jan 1, 1977·The Journal of Comparative Neurology·A D Loewy, R E Schader
Dec 11, 1990·Toxicology and Applied Pharmacology·R M LoPachin, A J Saubermann
Jan 1, 1985·Central Nervous System Trauma : Journal of the American Paralysis Association·J H LucasC R Gardner
Mar 1, 1985·Journal of the Neurological Sciences·A M MalbouissonG Allt
May 1, 1985·Journal of Neuropathology and Experimental Neurology·J B KirkpatrickG W Gross
Apr 13, 1973·Brain Research·J R Donat, H M Wiśniewski
Apr 14, 1970·Brain Research·R L Friede, A J Martinez
Nov 1, 1973·The Journal of Cell Biology·W W Schlaepfer, R P Bunge
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R B BorgensM J Cohen
Jan 1, 1994·Progress in Brain Research·W TetzlaffA M Bedard
Jun 1, 1994·Journal of Neurobiology·R B Campenot
Dec 29, 1995·European Journal of Pharmacology·R A Wallis, K L Panizzon
Jun 1, 1996·The European Journal of Neuroscience·K M Giehl, W Tetzlaff
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·C S EddlemanG D Bittner
Nov 14, 1997·Journal of Neurocytology·M J GrovesF Scaravilli
Jun 26, 1999·The European Journal of Neuroscience·M OudegaM B Bunge
Sep 23, 2000·Journal of Neuroscience Methods·I Fayaz, C H Tator
Dec 22, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L F BarrosT Hermosilla
Nov 26, 2003·Experimental Neurology·Donna M GeddesRobert S Cargill
Aug 7, 2004·Experimental Neurology·Gianluca Gallo
Sep 29, 2004·Microscopy Research and Technique·Gürkan Oztürk, Ender Erdoğan
Apr 21, 2006·Cellular and Molecular Neurobiology·Daria Guseva, Yuri Chelyshev
Dec 23, 2006·Journal of Neurotrauma·Anna N TaylorPaolo Prolo

❮ Previous
Next ❯

Citations

Feb 17, 2015·Molecular and Cellular Neurosciences·Mariana BrizuelaTracey C Dickson
Aug 9, 2018·Neurological Research·Ramazan ÜstünHasan Korkaya
Oct 11, 2017·Alternatives to Laboratory Animals : ATLA·Ashwin Kumaria
Jan 8, 2018·Turkish Journal of Medical Sciences·Aydın HimNureddin Cengiz
Aug 1, 2012·Neuropathology and Applied Neurobiology·G ÖztürkN Ayşit
Nov 12, 2018·Cellular and Molecular Neurobiology·Elif Kaval Oğuz, Gürkan Öztürk
Nov 28, 2020·International Journal of Nanomedicine·Elif Nur YilmazMehmet Hikmet Ucisik

❮ Previous
Next ❯

Software Mentioned

Axiovision
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.