Consequences of resistance evolution in a Cas9-based sex conversion-suppression gene drive for insect pest management.

Proceedings of the National Academy of Sciences of the United States of America
Mohammad KaramiNejadRanjbarErnst A Wimmer

Abstract

The use of a site-specific homing-based gene drive for insect pest control has long been discussed, but the easy design of such systems has become possible only with the recent establishment of CRISPR/Cas9 technology. In this respect, novel targets for insect pest management are provided by new discoveries regarding sex determination. Here, we present a model for a suppression gene drive designed to cause an all-male population collapse in an agricultural pest insect. To evaluate the molecular details of such a sex conversion-based suppression gene drive experimentally, we implemented this strategy in Drosophila melanogaster to serve as a safe model organism. We generated a Cas9-based homing gene-drive element targeting the transformer gene and showed its high efficiency for sex conversion from females to males. However, nonhomologous end joining increased the rate of mutagenesis at the target site, which resulted in the emergence of drive-resistant alleles and therefore curbed the gene drive. This confirms previous studies that simple homing CRISPR/Cas9 gene-drive designs will be ineffective. Nevertheless, by performing population dynamics simulations using the parameters we obtained in D. melanogaster and by adjusting the mod...Continue Reading

References

Apr 16, 1997·Molecular & General Genetics : MGG·B P DunckerV K Walker
Dec 17, 2002·Nature Biotechnology·Carsten Horn, Ernst A Wimmer
Jun 26, 2008·PloS One·Gyula TiminszkyAndreas G Ladurner
Jan 10, 2014·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·E Geuverink, L W Beukeboom
Jun 11, 2014·Nature Communications·Roberto GaliziAndrea Crisanti
Jul 19, 2014·Science·Kenneth A OyeJames P Collins
Jul 19, 2014·ELife·Kevin M EsveltGeorge M Church
May 15, 2015·Nature·Jeantine Lunshof
Aug 1, 2015·Science·Omar S AkbariJill Wildonger
Aug 2, 2015·Genetics·Robert L UncklessAndrew G Clark
Nov 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Valentino M GantzAnthony A James
Feb 16, 2016·Nature Reviews. Genetics·Jackson ChamperOmar S Akbari
Aug 4, 2016·Scientific Reports·Roberto GaliziAndrea Crisanti
Dec 13, 2016·Genetics·Robert L UncklessPhilipp W Messer
Apr 25, 2017·Science Advances·Charleston NobleMartin A Nowak
Aug 11, 2017·Proceedings. Biological Sciences·Thomas A A ProwsePaul Thomas
May 8, 2018·Proceedings of the National Academy of Sciences of the United States of America·Jackson ChamperPhilipp W Messer

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Citations

Nov 28, 2018·Ecology Letters·David N ReznickJoseph Travis
Dec 1, 2018·Biology Open·Alexander NashNikolai Windbichler
Jan 23, 2019·ELife·Jackson ChamperPhilipp W Messer
Dec 29, 2019·G3 : Genes - Genomes - Genetics·Nikolay P KandulOmar S Akbari
Feb 25, 2020·Archives of Insect Biochemistry and Physiology·Maria-Eleni Gregoriou, Kostas D Mathiopoulos
Sep 25, 2020·Journal of Evolutionary Biology·Tom A R PriceAnna K Lindholm
Sep 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·Georg OberhoferBruce A Hay
Nov 24, 2018·Scientific Reports·Yao Yan, Gregory C Finnigan
Feb 11, 2020·Frontiers in Bioengineering and Biotechnology·Heidi J Mitchell, Detlef Bartsch
Feb 29, 2020·Nature Communications·Jackson ChamperPhilipp W Messer
Dec 29, 2019·Scientific Reports·Matthew G Heffel, Gregory C Finnigan
Sep 11, 2020·Evolutionary Applications·Kristina Karlsson GreenÅsa Lankinen
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Yutao Xiao, Kongming Wu
Feb 9, 2020·The Journal of Experimental Biology·Robyn R RabanOmar S Akbari
Sep 16, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jackson ChamperPhilipp W Messer
Sep 11, 2020·Evolutionary Applications·Virginie Courtier-OrgogozoChristophe Boëte
Mar 14, 2020·BMC Biology·Jackson ChamperPhilipp W Messer
Nov 27, 2020·Scientific Reports·Matthew P EdgingtonLuke Alphey
Dec 4, 2020·PLoS Neglected Tropical Diseases·David Navarro-PayáLuke Alphey
Oct 10, 2020·Annual Review of Entomology·Bruce A HayMing Guo
Dec 29, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Josef ZapletalZach N Adelman
Mar 6, 2021·ELife·Nikolay P KandulOmar S Akbari
Jul 30, 2021·Nature Communications·Andrew HammondAndrea Crisanti
Dec 1, 2021·Proceedings of the National Academy of Sciences of the United States of America·Georg OberhoferBruce A Hay

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