Consequences of transient focal cerebral ischaemia for second messenger and neurotransmitter binding in the rat: quantitative autoradiographic analysis of forskolin, dopamine D1 receptor binding and cerebral blood flow changes

The European Journal of Neuroscience
G GartshoreI M Macrae

Abstract

In order to study the consequences of reperfusion for ischaemic brain injury, quantitative ligand binding autoradiography was carried out in a model of reversible focal cerebral ischaemia. Endothelin-1 applied to the abluminal surface of the middle cerebral artery in anaesthetized Sprague-Dawley rats induced severe focal ischaemia and subsequent reperfusion (assessed by blood flow tracers [99mTc]HMPAO and [14C]iodoantipyrine respectively) by 2 h after insult. Ligand binding autoradiography on consecutive sections demonstrated these blood flow changes to be associated with a significant reduction in forskolin binding throughout the middle cerebral artery territory (e.g. 25% in parietal cortex, 11% in dorsolateral caudate nucleus). The most marked losses in forskolin binding were in areas where ischaemia was severe and reperfusion was poor. However, the same changes in cerebral blood flow had no significant effect on D1 dopamine receptor binding (e.g. < 2% reduction in the caudate nucleus). These data demonstrate that ligand binding characteristics are significantly affected as early as 2 h after insult, with evidence of differential sensitivity for forskolin and D1 dopamine binding. With regard to the consequences of reperfusion...Continue Reading

References

Mar 1, 1991·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·K TanakaB Mihara
Nov 1, 1990·Archives of Neurology·J M Hallenbeck, A J Dutka
Mar 1, 1990·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M J Robinson, J McCulloch
Jan 1, 1989·Stroke; a Journal of Cerebral Circulation·E Z LongaR Cummins
Nov 1, 1989·Trends in Pharmacological Sciences·A LaurenzaK B Seamon
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P BrayM Nirenberg
Dec 1, 1988·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·N A LassenO B Paulson
Dec 1, 1988·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Y YonekuraN A Lassen
Jan 15, 1988·European Journal of Biochemistry·S Mollner, T Pfeuffer
Dec 30, 1985·Brain Research·D R GehlertJ K Wamsley
Jan 1, 1982·Journal of Neurosurgery·J P Kapp, R R Smith
Jan 1, 1981·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A TamuraG M Teasdale
Nov 1, 1994·Stroke; a Journal of Cerebral Circulation·J AronowskiJ C Grotta
Mar 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·I M MacraeJ McCulloch
Feb 1, 1991·Molecular and Cellular Neurosciences·S L DrinnanS R Vincent

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.