Conservation analysis predicts in vivo occupancy of glucocorticoid receptor-binding sequences at glucocorticoid-induced genes.

Proceedings of the National Academy of Sciences of the United States of America
Alex Y SoKeith R Yamamoto

Abstract

The glucocorticoid receptor (GR) interacts with specific GR-binding sequences (GBSs) at glucocorticoid response elements (GREs) to orchestrate transcriptional networks. Although the sequences of the GBSs are highly variable among different GREs, the precise sequence within an individual GRE is highly conserved. In this study, we examined whether sequence conservation of sites resembling GBSs is sufficient to predict GR occupancy of GREs at genes responsive to glucocorticoids. Indeed, we found that the level of conservation of these sites at genes up-regulated by glucocorticoids in mouse C3H10T1/2 mesenchymal stem-like cells correlated directly with the extent of occupancy by GR. In striking contrast, we failed to observe GR occupancy of GBSs at genes repressed by glucocorticoids, despite the occurrence of these sites at a frequency similar to that of the induced genes. Thus, GR occupancy of the GBS motif correlates with induction but not repression, and GBS conservation alone is sufficient to predict GR occupancy and GRE function at induced genes.

Associated Datasets

May 25, 2010·Brian J FeldmanMitra Manuchehri

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Citations

Jan 15, 2013·Endocrine·Johan Arild EvangJens Petter Berg
Dec 27, 2012·Molecular and Cellular Endocrinology·Dariusz RatmanKarolien De Bosscher
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Andrea R Daniel, Carol A Lange
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Alex Y -L SoBrian J Feldman
Nov 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Neil S HoldenRobert Newton
Jan 14, 2011·The Journal of Biological Chemistry·Maria Jose CostaBrian J Feldman
Aug 27, 2011·The Journal of Biological Chemistry·Alejandro A HidalgoDonald L Trump
Jul 10, 2008·American Journal of Physiology. Endocrinology and Metabolism·David S WaddellSue C Bodine
Jan 26, 2010·PloS One·Marinus F van BatenburgOnno C Meijer
Dec 1, 2011·Epigenomics·Christopher D Green, Jing-Dong J Han
Nov 19, 2013·BMC Genomics·Shan ZhongZiv Bar-Joseph
Jun 21, 2013·Cellular and Molecular Life Sciences : CMLS·Karolien De BosscherGuy Haegeman
Jan 15, 2013·Trends in Endocrinology and Metabolism : TEM·John M Busillo, John A Cidlowski
Jan 4, 2012·Pharmacology & Therapeutics·Andrew R Clark, Maria G Belvisi
May 21, 2011·Clinical Endocrinology·Johan Arild EvangJens Bollerslev
Apr 20, 2011·The FEBS Journal·Malgorzata WienchGordon L Hager
Jul 11, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Nancy H IngThomas H Welsh
Apr 20, 2010·Molecular and Cellular Endocrinology·Agnes E Coutinho, Karen E Chapman
Jan 5, 2011·Trends in Endocrinology and Metabolism : TEM·Guilherme M SantosJohn W R Schwabe
Apr 1, 2015·Translational Psychiatry·J BrockhurstD M Lyons
Sep 10, 2011·Angewandte Chemie·Miki ImanishiHitoshi Okamura
Apr 30, 2014·Molecular and Cellular Biology·Melanie E PefferDonald B DeFranco
Dec 20, 2008·Molecular Endocrinology·Karolien De Bosscher, Guy Haegeman
May 31, 2019·Nucleic Acids Research·Nicholas V ParsonnetDeborah S Wuttke
May 24, 2019·Journal of Neuroendocrinology·Lisa T C M van WeertOnno C Meijer
Jun 7, 2019·The Journal of Biological Chemistry·Maria G PetrilloJohn A Cidlowski
Nov 20, 2016·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Rachel J EclovDeanna L Kroetz

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