Conservation and diversity of eukaryotic translation initiation factor eIF3.

The Journal of Biological Chemistry
K AsanoJ W Hershey

Abstract

The largest of the mammalian translation initiation factors, eIF3, consists of at least eight subunits ranging in mass from 35 to 170 kDa. eIF3 binds to the 40 S ribosome in an early step of translation initiation and promotes the binding of methionyl-tRNAi and mRNA. We report the cloning and characterization of human cDNAs encoding two of its subunits, p110 and p36. It was found that the second slowest band during polyacrylamide gel electrophresis of eIF3 subunits in sodium dodecyl sulfate contains two proteins: p110 and p116. Analysis of the cloned cDNA encoding p110 indicates that its amino acid sequence is 31% identical to that of the yeast protein, Nip1. The p116 cDNA was cloned and characterized as a human homolog of yeast Prt1, as described elsewhere (Methot, N., Rom, E., Olsen, H., and Sonenberg, N. (1997) J. Biol. Chem. 272, 1110-1116). p36 is a WD40 repeat protein, which is 46% identical to the p39 subunit of yeast eIF3 and is identical to TRIP-1, a phosphorylation substrate of the TGF-beta type II receptor. The p116, p110, and p36 subunits localize on 40 S ribosomes in cells active in translation and co-immunoprecipitate with affinity-purified antibodies against the p170 subunit, showing that these proteins are integ...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·W C Merrick
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R Benne, J W Hershey
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·Z GuD S Goldfarb
Jan 1, 1990·Archives of Biochemistry and Biophysics·S C MilburnJ W Hershey
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R H AebersoldS B Kent
Feb 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·L H Hartwell, C S McLaughlin
Nov 18, 1983·Science·R A Young, R W Davis
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·M B SoaresA Efstratiadis
Jan 25, 1996·Nature·D G LambrightP B Sigler

❮ Previous
Next ❯

Citations

May 30, 1998·Protein Science : a Publication of the Protein Society·L Aravind, C P Ponting
Jan 29, 2013·Cellular and Molecular Life Sciences : CMLS·Roberta MarchioneJean-Luc Lenormand
Jan 1, 1997·Cellular Signalling·K Williams
Jun 30, 1998·Trends in Biochemical Sciences·K Hofmann, P Bucher
Apr 4, 2003·The International Journal of Biochemistry & Cell Biology·Vered Maytal-KivityMichael H Glickman
Aug 10, 2001·Trends in Plant Science·T KimD A Chamovitz
Sep 22, 2001·RNA·D Cao, R Parker
Apr 13, 2012·Cell Death & Disease·B M WalterV Wixler
Feb 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Susana T ValenteStephen P Goff
Jun 13, 2006·The Journal of Biological Chemistry·Aaron K LeFebvreRobert E Rhoads
May 6, 1999·The EMBO Journal·C M FletcherG Wagner
Aug 13, 2004·Molecular Plant-microbe Interactions : MPMI·Nikos HontzeasBernard R Glick
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·K W Buck
Sep 3, 2011·Microbiology and Molecular Biology Reviews : MMBR·Alan G Hinnebusch
Sep 25, 2003·Annual Review of Plant Biology·Giovanna Serino, Xing-Wang Deng
Jan 21, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Amsaveni RamachandranAnne George
Nov 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hong JiJennifer A Doudna
Mar 5, 1999·Molecular Plant-microbe Interactions : MPMI·M L van BuurenM J Harrison
Jun 5, 2004·Genomics·Jerald P RadichPeter S Linsley
Jul 7, 2005·The Plant Journal : for Cell and Molecular Biology·Hashimul EhsanSteven D Clouse
Apr 19, 2003·Experimental Dermatology·Alfonso BaldiMarco G Paggi
Mar 31, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Diana Metz-EstrellaJ Edward Puzas
Aug 22, 2006·Trends in Biochemical Sciences·Alan G Hinnebusch
Aug 2, 2005·Current Opinion in Plant Biology·Jianming Li
Feb 27, 2004·Biochemical and Biophysical Research Communications·Jhy-Shrian HuangYuh-Shan Jou
Apr 10, 2003·Biochemical and Biophysical Research Communications·Jung Min HanSunghoon Kim

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.