Conservation of bacterial protein synthesis machinery: initiation and elongation in Mycobacterium smegmatis

Biochemistry
Christian BruellErik C Böttger

Abstract

Most of our understanding of ribosome function is based on experiments utilizing translational components from Escherichia coli. It is not clear to which extent the details of translation mechanisms derived from this single organism are true for all bacteria. Here we investigate translation factor-dependent reactions of initiation and elongation in a reconstituted translation system from a Gram-positive bacterium Mycobacterium smegmatis. This organism was chosen because mutations in rRNA have very different phenotypes in E. coli and M. smegmatis, and the docking site for translational GTPases, the L12 stalk, is extended in the ribosomes from M. smegmatis compared to E. coli. M. smegmatis genes coding for IF1, IF2, IF3, EF-G, and EF-Tu were identified by sequence alignments; the respective recombinant proteins were prepared and studied in a variety of biochemical and biophysical assays with M. smegmatis ribosomes. We found that the activities of initiation and elongation factors and the rates of elemental reactions of initiation and elongation of protein synthesis are remarkably similar with M. smegmatis and E. coli components. The data suggest a very high degree of conservation of basic translation mechanisms, probably due to c...Continue Reading

Citations

Jan 24, 2013·Nature Communications·Iakov I DavydovMarina V Rodnina
Oct 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yuan LinShuyi Si
Jul 20, 2011·Antimicrobial Agents and Chemotherapy·Rashid AkbergenovErik C Böttger
Sep 6, 2012·Antimicrobial Agents and Chemotherapy·Sumantha SalianErik C Böttger
Jan 30, 2014·Nature Communications·Déborah Perez-FernandezErik C Böttger

Related Concepts

Bacterial Proteins
Alkalescens-Dispar Group
Peptide Elongation Factor Tu
Elongation Factor
Plasma Protein Binding Capacity
Phenylalanine-Specific tRNA
Protein Biosynthesis
Homologous Sequences, Amino Acid
Mycobacterium smegmatis
Peptide Elongation Factor G

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.