Conservation of epidermal growth factor receptor function in the human parasitic helminth Schistosoma mansoni.

The Journal of Biological Chemistry
Jérôme VicogneColette Dissous

Abstract

The epidermal growth factor receptor (EGF-R) plays an important role in development and cell differentiation, and homologues of EGF-R have been identified in a broad range of vertebrate and invertebrate organisms. This work concerns the functional characterization of SER, the EGF-R-like molecule previously identified in the helminth parasite Schistosoma mansoni. Transactivation assays performed in epithelial Madin-Darby canine kidney cells co-transfected with SER and a Ras-responsive reporter vector indicated that SER was able to trigger a Ras/ERK pathway in response to human epidermal growth factor (EGF). These results were confirmed in Xenopus oocytes showing that human EGF induced meiosis reinitiation characterized by germinal vesicle breakdown in SER-expressing oocytes. Germinal vesicle breakdown induced by EGF was dependent on receptor kinase activity and shown to be associated with phosphorylation of SER and of downstream ERK proteins. (125)I-EGF binding experiments performed on SER-expressing oocytes revealed high affinity (2.9 x 10(-9) M) of the schistosome receptor for human EGF. Phosphorylation of the native SER protein present in S. mansoni membranes was also shown to occur upon binding of human EGF. These data demon...Continue Reading

References

Jul 1, 1992·Molecular and Biochemical Parasitology·C B ShoemakerL D Stein
Apr 20, 1990·Cell·A Ullrich, J Schlessinger
May 1, 1985·Molecular and Biochemical Parasitology·R J PierceA Capron
Apr 1, 1995·Developmental Biology·O HaccardJ L Maller
May 1, 1997·Trends in Genetics : TIG·R Schweitzer, B Z Shilo
Nov 23, 2000·Molecular and Biochemical Parasitology·M J BeallE J Pearce
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·Y Yarden, M X Sliwkowski
Mar 17, 2001·Nature Reviews. Neuroscience·T R Insel, L J Young
Sep 22, 2001·Endocrine-related Cancer·E ZwickA Ullrich
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 19, 2002·Parasitology Research·Jean-François CoppinColette Dissous
Jan 30, 2003·Molecular and Biochemical Parasitology·Jérôme VicogneColette Dissous
Mar 22, 2003·Experimental Cell Research·Robert N JorissenAntony W Burgess

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Citations

Feb 18, 2010·Parasitology Research·Jinbiao PengJiaojiao Lin
Oct 21, 2011·The Journal of Biological Chemistry·Svenja BeckmannChristoph G Grevelding
Jul 28, 2009·Annual Review of Genomics and Human Genetics·Ze-Guang HanZhu Chen
May 10, 2011·BMC Genomics·Luiza F AndradeGuilherme Oliveira
May 23, 2013·PLoS Neglected Tropical Diseases·Mathieu VanderstraeteColette Dissous
Jun 6, 2014·PLoS Neglected Tropical Diseases·Andreas SchubertKlaus Brehm
Mar 31, 2010·PloS One·Hong YouDonald P McManus
Mar 17, 2011·PloS One·Philipp LudinPascal Mäser
May 31, 2014·PLoS Pathogens·Mathieu VanderstraeteColette Dissous
Jun 20, 2014·PLoS Neglected Tropical Diseases·Luiza Freire de AndradeGuilherme Oliveira
Jul 10, 2012·International Journal for Parasitology·Hong YouDonald P McManus
Oct 6, 2010·Trends in Parasitology·Colette Dissous, Christoph G Grevelding
Jul 27, 2007·International Journal for Parasitology·Yutao YanColette Dissous
Mar 3, 2009·Journal of Basic Microbiology·Wang Ting-An, Zhuge Hong-Xiang
Jan 25, 2005·Molecular and Biochemical Parasitology·Markus SpiliotisKlaus Brehm
Mar 9, 2006·Molecular and Biochemical Parasitology·Naji KhayathColette Dissous
Dec 14, 2005·Parasitology International·Klaus BrehmMatthias Frosch
Jul 24, 2007·Experimental Parasitology·Philip T LoverdeAndrew Hinck
Jun 8, 2007·Experimental Parasitology·Jürgen KnoblochChristoph G Grevelding
Apr 19, 2015·Parasites & Vectors·Francois Olivier HebertNadia Aubin-Horth
Jul 7, 2009·Current Opinion in Microbiology·Philip T LoVerdeGuilherme Oliveira
May 28, 2011·Experimental Parasitology·Nadege GouignardColette Dissous
May 23, 2014·The Korean Journal of Parasitology·Junling HouXuepeng Cai

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